Latest Cover

Online Office

Contact Us

Issue:ISSN 1000-7083
          CN 51-1193/Q
Director:Sichuan Association for Science and Technology
Sponsored by:Sichuan Society of Zoologists; Chengdu Giant Panda Breeding Research Foundation; Sichuan Association of Wildlife Conservation; Sichuan University
Address:College of Life Sciences, Sichuan University, No.29, Wangjiang Road, Chengdu, Sichuan Province, 610064, China
Fax:+86-28-85410485 &
Your Position :Home->Past Journals Catalog->2018 Vol.37 No.4

Taxonomical Status of Eremias arguta from Bole City and Ili River Valley, Xinjiang Uyghur Autonomous Region, China
Author of the article:GONG Xiong1,2, LIU Jinlong1,2, ZHOU Tianhe1,2, SONG Qi1,2, GUO Xianguang1*
Author's Workplace:1. Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China;
2. University of Chinese Academy of Sciences, Beijing 100049, China
Key Words:Eremias arguta; Ili River Valley; intraspecies systematics; morphology; cytochrome b gene; molecular phylogeny
Abstract:The steppe racerunner (Eremias arguta) is one of the most widespread species of the Eurasian racerunners (genus Eremias), inhabiting steppes and semi-deserts of eastern Europe and central Asia from Romania to Mongolia and western China. Historically, 6 subspecies are recognized, and with 2 subspecies occurring in China; however, the taxonomical status of some subspecies is still controversial due in part to their high morphological variability. The present study deals with the intraspecies systematics of 28 samples representing 4 populations in Bole city and Ili River Valley, Xinjiang Uyghur Autonomous Region, China, through morphological comparison and phylogenetic analysis based on mitochondrial DNA cytochrome b (Cyt b) gene. Cyt b gene sequences of E. arguta available in the GenBank were compiled for comparison with the obtained haplotypes. Tree-based subspecies delimitation was achieved by using maximum parsimony, maximum likelihood and Bayesian phylogenetic analyses. Bayes factors (BF) were used to test the competing hypotheses for E. arguta by comparing the unconstrained Bayesian tree topology to Bayesian trees with ‘hard’ constraints. The morphological features of steppe racerunner population in Bole city matched the description of those from the Ili River Valley, with specific dorsal pattern characterized by the presence of bright blue or green ocelli in breeding males. Phylogenetic trees congruently indicated that the haplotypes from Bole city belong to the Ili River Valley clade, which represents a separate lineage with undescribed subspecies (E. arguta ssp.). The 3 hypotheses that populations from the Ili River Valley belong to the subspecies E. a. arguta, or E. a. potanini, or E. a. uzbekistanica were rejected with very strong evidence (2lnBF>10), respectively. The Ili River Valley clade is most likely the sister taxon to E. a. uzbekistanica and E. a. darevskii; meanwhile, there is no reliable evidence to reject the alternative hypothesis that E. arguta sister to the remaining lineages of E. a. arguta (2 < 2lnBF < 6). In addition, the reciprocal monophyly of E. a. potanini and E. a. arguta was recovered albeit with low bootstrap values and moderate Bayesian posterior probabilities. An alternative topology with samples of E. a. potanini nested within the E. a. arguta clade was not rejected with very strong evidence (6 < 2lnBF < 10). In conclusion, our results demonstrate that:(i) the steppe racerunner in Bole city is not E. a. potanini, but belongs to the Ili River Valley clade and thus represents a new record of E. arguta ssp. in Junggar Basin, Xinjiang, China; (ii) the populations from the Ili River Valley should not be classified as E. a. arguta or E. a. potanini or E. a. uzbekistanica, but belong to a cryptic clade (an undescribed subspecies); (iii) the phylogenetic placement of the novel clade (subspecies) is unclear and needing further clarification with integrative taxonomic approach. Finally, in combination with morphological variability, the validity of the E. a. potanini subspecies was discussed.
2018,37(4): 387-399 收稿日期:2018-01-07
戴鑫, 陈彬, 张正卫, 等. 2006. 中国八种麻蜥(蜥蜴科,麻蜥属)形态学研究[J]. 动物分类学报, 31(4):697-708.
郭宪光, 陈达丽, 万宏富, 等. 2010. 麻蜥属Eremias的系统学研究进展[J]. 四川动物, 29(4):665-672.
刘金龙, 原秀云, 周天和, 等. 2014. 新疆天山以北发现爬行动物虫纹麻蜥[J]. 动物学杂志, 49(3):423-427.
赵尔宓, 赵肯堂, 周开亚, 等. 1999. 中国动物志 爬行纲(第二卷) 有鳞目 蜥蜴亚目[M]. 北京:科学出版社:226-228.
Altschul SF, Madden TL, Schaffer AA, et al. 1997. Gapped BLAST and PSI-BLAST:a new generation of protein database search programs[J]. Nucleic Acids Research, 25(17):3389-3402.
Burbrink FT, Lawson R, Slowinski JB. 2000. Mitochondrial DNA phylogeography of the polytypic north American rat snake (Elaphe obsoleta):a critique of the subspecies concept[J]. Evolution, 54(6):2107-2118.
Chernov SA. 1934. On the subspecies and distribution of the steppe racerunner[J]. Proceedings of the USSR Academy of Sciences, Biological Series, 3(8-9):666-668 (in Russian).
Dujsebayeva ТN, Belyalov OV, Orlova VF, et al. 2007. Unusual find of the steppe-runner, Eremias arguta (Pallas, 1773) with blue spots in southeast of Kazakhstan[J]. Terra, 2007:118-121.
Gouy M, Guindon S, Gascuel O. 2010. SeaView version 4:a multiplatform graphical user interface for sequence alignment and phylogenetic tree building[J]. Molecular Biology & Evolution, 27(2):221-224.
Guo X, Dai X, Chen D, et al. 2011. Phylogeny and divergence times of some racerunner lizards (Lacertidae:Eremias) inferred from mitochondrial 16S rRNA gene segments[J]. Molecular Phylogenetics & Evolution, 61(2):400-412.
Kass RE, Raferty AE. 1995. Bayes factors[J]. Journal of the American Statistical Association, 90(430):773-795.
Kumar S, Stecher G, Tamura K. 2016. MEGA7:molecular evolutionary genetics analysis version 7.0 for bigger datasets[J]. Molecular Biology & Evolution, 33(7):1870-1874.
Lanfear R, Frandsen PB, Wright AM, et al. 2017. PartitionFinder 2:new methods for selecting partitioned models of evolution for molecular and morphological phylogenetic analyses[J]. Molecular Biology & Evolution, 34(3):772-773.
Librado P, Rozas J. 2009. DnaSP v5:a software for comprehensive analysis of DNA polymorphism data[J]. Bioinformatics, 25(11):1451-1452.
Newton MA, Raftery AE. 1994. Approximate Bayesian inference with the weighted likelihood bootstrap[J]. Journal of the Royal Statistical Society, Series B:Methodological, 56(1):3-48.
Orlova VF, Chirikova MA, Pavlinov NJ. 2012. Steppe racerunner, Eremias arguta (Pallas, 1773) (Sauria, Lacertidae) in the eastern part of its range:morphological variability and taxonomic status of populations[J]. Zoological Journal, 91(11):1366-1376 (in Russian with English abstract).
Padial JM, Miralles A, De la Riva I, et al. 2010. The integrative future of taxonomy[J]. Frontiers in Zoology, 7(1):1-14.
Pavlicev M, Mayer W. 2009. Fast radiation of the subfamily Lacertinae (Reptilia:Lacertidae):history or methodical artefact?[J]. Molecular Phylogenetics & Evolution, 52(3):727-734.
Poyarkov NJ, Orlova VF, Chirikova MA. 2014. The mitochondrial phylogeography and intraspecific taxonomy of the steppe racerunner,Eremias arguta (Pallas) (Lacertidae:Sauria, Reptilia), reflects biogeographic patterns in middle Asia[J]. Zootaxa, 3895(2):208-223.
Rambaut A, Drummond AJ. 2009. Tracer MCMC trace analysis tool, version v1.5.0 2003-2009[M]. Computer program distributed by the authors.[2017-12-10].
Ronquist F, Teslenko M, van Der Mark P, et al. 2012. MrBayes 3.2:efficient Bayesian phylogenetic inference and model choice across a large model space[J]. Systematic Biology, 61(3):539-542.
Schwarz G. 1978. Estimating the dimension of a model[J]. Annals of Statistics, 6(2):461-464.
Sindaco R, Jeremenko VK, Venchi A. 2008. The reptiles of the western Palearctic:annotated checklist and distributional Atlas of the turtles, crocodiles, amphisbaenians and lizards of Europe, North Africa, Middle East and Central Asia[M]. Latina:Edizioni Belvedere:228, 362.
Song H, Buhay JE, Whiting MF, et al. 2008. Many species in one:DNA barcoding overestimates the number of species when nuclear mitochondrial pseudogenes are coamplified[J]. Proceedings of the National Academy of Sciences of the United States of America, 105(36):13486-13491.
Stamatakis A. 2006. RAxML-VI-HPC:maximum likelihood-based phylogenetic analyses with thousands of taxa and mixed models[J]. Bioinformatics, 22(21):2688-2690.
Suchard MA, Weiss RE, Sinsheimer JS. 2001. Bayesian selection of continuous-time Markov chain evolutionary models[J]. Molecular Biology & Evolution, 18(6):1001-1013.
Swofford DL. 2003. PAUP*:phylogenetic analysis using parsimony (*and other methods), version 4.0b10[M]. Sunderland, Massachusetts:Sinauer Associates.
Szczerbak NN, Neruchev VV, Okulova NM, et al. 1993. Taxonomy, geographic variation and interspecific structure[M]//Szczerbak NN. Eremias arguta. Kiev:Naukova Dumka:22-34 (in Russian).
Szczerbak NN. 1974. Yashchurki Palearktiki (Eremias lizards of the Palearctic)[M]. Kiev:Naukova Dumka:146-180 (in Russian).
Szczerbak NN. 2003. Guide to the reptiles of the eastern Palearctic[M]. Malabar, Florida:Krieger Publishing Company:79-80.
Torstrom SM, Pangle KL, Swanson BJ. 2014. Shedding subspecies:the influence of genetics on reptile subspecies taxonomy[J]. Molecular Phylogenetics & Evolution, 76:134-143.
Yakovleva ID. 1964. Reptiles of Kirgizia[M]. Frunze:Academy of Sciences of the Kirghiz SSR Press:138-167 (in Russian).
Zhao Q, Liu H, Luo L, et al. 2011. Comparative population genetics and phylogeography of two lacertid lizards (Eremias argus and E. brenchleyi) from China[J]. Molecular Phylogenetics & Evolution, 58(3):478-491.
CopyRight©2018 Editorial Office of Sichuan Journal of Zoology