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Issue:ISSN 1000-7083
          CN 51-1193/Q
Director:Sichuan Association for Science and Technology
Sponsored by:Sichuan Society of Zoologists; Chengdu Giant Panda Breeding Research Foundation; Sichuan Association of Wildlife Conservation; Sichuan University
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Your Position :Home->Past Journals Catalog->2018 Vol.37 No.4

The Application of Extracting DNA from Skin Swabbing in Amphibians
Author of the article:AI Yongbin1, YANG Xusheng2,3, PENG Weihua1, CHEN Yunmei1, XU Liangyan1, LUO Jian1, XIA Yun2, ZENG Xiaomao2*
Author's Workplace:1. Administrative Office of Shenguozhuang Provincial Giant Panda Nature Reserve, Yuexi, Sichuan Province 616650, China;
2. Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China;
3. University of Chinese Academy of Sciences, Beijing 100049, China
Key Words:skin swab sampling; amphibian; conservation genetics
Abstract:Compared to other sampling methods, non-invasive sampling can minimize the damage to animals, so it has important value for animal protection. There is only a few reports on skin swabbing in a limited number of amphibian species, and the sampling methods are all performed in laboratory environment and are not suitable for application in the wild fields. In this study, Shenguozhuang Provincial Giant Panda Nature Reserve in Sichuan was selected as the research area. The skin swabbing method was used on wild populations of 11 amphibian species to test DNA yields and quality. Finally, the DNA was successfully obtained and multiple of genetic markers were amplified. Based on PCR performance, DNA yields and sampling considerations, the method of skin swab sampling was confirmed to work well to obtain DNA without harming the amphibian. Therefore, skin swabbing is a reliable method for obtaining genetic data from endangered amphibian without jeopardizing their survival.
2018,37(4): 373-380 收稿日期:2017-09-23
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