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Issue:ISSN 1000-7083
          CN 51-1193/Q
Director:Sichuan Association for Science and Technology
Sponsored by:Sichuan Society of Zoologists; Chengdu Giant Panda Breeding Research Foundation; Sichuan Association of Wildlife Conservation; Sichuan University
Address:College of Life Sciences, Sichuan University, No.29, Wangjiang Road, Chengdu, Sichuan Province, 610064, China
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Your Position :Home->Past Journals Catalog->2018 Vol.37 No.3

Genetic Diversity of Offsprings Derived from Wild and Cultivated Populations of Macaca thibetana
Author of the article:NIU Lihong1, ZHOU Liang1, YANG Fan1, WU Aiming1, FU Guoqiang1, HOU Linlin1, LI Xiao2, DOU Kefeng2, CHEN Hui1, ZHANG Xinshang1*
Author's Workplace:1. Institute of Laboratory Animal Sciences, Sichuan Academy of Medical Sciences and Sichuan Provincial People's Hospital, Chengdu 610212, China;
2. Department of Hepatobiliary Surgery, Xijing Hospital, the Fourth Military Medical University, Xi'an 710032, China
Key Words:Macaca thibetana; generation; mitochondrial DNA; D-loop; genetic diversity
Abstract:To evaluate the genetic variation of offsprings derived from wild and cultivated populations of Macaca thibetana, we analyzed the whole sequence of mitochondrial DNA D-loop region in the 102 individuals of 2 generations of wild (Jinyang region) and cultivated populations (Mabian region). The whole length of the region sequence was 1 091 bp and 115 variable sites were detected. The 102 individuals presented 66 haplotypes, and the 2 generations of Mabian and Jinyang population shared 2 and 1 haplotype with their parents, respectively. The nucleotide polymorphisms of the 2 generations of Mabian population were 0.004 77 and 0.004 09, while those in Jinyang population were 0.002 30 and 0.002 78, indicating no significant difference of genetic diversity occurred between the offsprings and parents of M. thibetana. Genetic distances between the 2 generations of Mabian and Jinyang populations were 0.024 09 and 0.002 55, indicating no significant differentiation happened in the 2 generations in wild or in cultivated situation. NJ tree based on the whole length of D-loop showed 2 independent clades existed in Mabian and Jinyang populations with different generations crossed each other, this demonstrated that neither the random mating nor the artificial selection pressure exerted a significant influence on the genetic structure of the progeny. This study was the first time to use the whole length of mitochondrial DNA D-loop region to analyze the genetic diversity of wild and cultivated Macaca thibetana populations and their offsprings. Our data provide basic support for the standardization of genetic control of experimental animals.
2018,37(3): 280-284,290 收稿日期:2017-12-13
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