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Issue:ISSN 1000-7083
          CN 51-1193/Q
Director:Sichuan Association for Science and Technology
Sponsored by:Sichuan Society of Zoologists; Chengdu Giant Panda Breeding Research Foundation; Sichuan Association of Wildlife Conservation; Sichuan University
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Your Position :Home->Past Journals Catalog->2018 Vol.37 No.3

Gene Sequences of COⅠ and Cyt b of Ardea insignis and Molecular Phylogenetic Analysis of Genus Ardea
Author of the article:CHEN Xi1, WANG Bin2, LUO Xu1*
Author's Workplace:1. State Forestry Administration Key Laboratory of Biodiversity Conservation in Southwest China, Southwest Forestry University, Kunming 650224, China;
2. Lushui Management Bureau of Gaoligongshan National Nature Reserve, Lushui, Yunnan Province 673100, China
Key Words:Ardea insignis; COⅠ gene; Cyt b gene; phylogenetic tree
Abstract:Ardea insignis is a rare species and listed as a critically endangered species in the IUCN red list of threatened species. It belongs to genus Ardea of family Ardeidae. There are very few studies carried on this species. We obtained COⅠ and Cyt b gene sequences of A. insignis using PCR technology. The length of the Cyt b gene was 1 163 bp, and the A+T content (51.7%) was higher than that of C+G (48.3%). The length of the COⅠ gene was 722 bp, and the A+T content (52.5%) was also higher than that of C+G (47.5%). The third site of both genes showed the most remarkable base bias. Combined with the sequences of the other 6 Ardea species from GenBank, the phylogenetic tree of genus Ardea was constructed. The result showed that 7 species of genus Ardea formed a monophyletic clade with the posterior support at 96%. A. alba and A. insignis were found at the basal position of this clade, indicating their early diverge. Based on the genetic analysis, A. purpurea was considered to be the most closely related species to A. insignis.
2018,37(3): 268-273 收稿日期:2017-11-10
DOI:10.11984/j.issn.1000-7083.20170353
分类号:Q959.7
基金项目:环保部生物多样性示范观测项目(2016-2017);云南省极小种群物种拯救保护项目(2016)
作者简介:陈熙(1993-),女,硕士研究生,研究方向:野生动植物保护与利用,E-mail:18387158232@163.com
*通讯作者:罗旭,副教授,研究方向:鸟类进化、生态与保护,E-mail:xu_luo@aliyun.com
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