Latest Cover

Online Office

Contact Us

Issue:ISSN 1000-7083
          CN 51-1193/Q
Director:Sichuan Association for Science and Technology
Sponsored by:Sichuan Society of Zoologists; Chengdu Giant Panda Breeding Research Foundation; Sichuan Association of Wildlife Conservation; Sichuan University
Address:College of Life Sciences, Sichuan University, No.29, Wangjiang Road, Chengdu, Sichuan Province, 610064, China
Fax:+86-28-85410485 &
Your Position :Home->Past Journals Catalog->2018 Vol.37 No.3

Gene Sequences of COⅠ and Cyt b of Ardea insignis and Molecular Phylogenetic Analysis of Genus Ardea
Author of the article:CHEN Xi1, WANG Bin2, LUO Xu1*
Author's Workplace:1. State Forestry Administration Key Laboratory of Biodiversity Conservation in Southwest China, Southwest Forestry University, Kunming 650224, China;
2. Lushui Management Bureau of Gaoligongshan National Nature Reserve, Lushui, Yunnan Province 673100, China
Key Words:Ardea insignis; COⅠ gene; Cyt b gene; phylogenetic tree
Abstract:Ardea insignis is a rare species and listed as a critically endangered species in the IUCN red list of threatened species. It belongs to genus Ardea of family Ardeidae. There are very few studies carried on this species. We obtained COⅠ and Cyt b gene sequences of A. insignis using PCR technology. The length of the Cyt b gene was 1 163 bp, and the A+T content (51.7%) was higher than that of C+G (48.3%). The length of the COⅠ gene was 722 bp, and the A+T content (52.5%) was also higher than that of C+G (47.5%). The third site of both genes showed the most remarkable base bias. Combined with the sequences of the other 6 Ardea species from GenBank, the phylogenetic tree of genus Ardea was constructed. The result showed that 7 species of genus Ardea formed a monophyletic clade with the posterior support at 96%. A. alba and A. insignis were found at the basal position of this clade, indicating their early diverge. Based on the genetic analysis, A. purpurea was considered to be the most closely related species to A. insignis.
2018,37(3): 268-273 收稿日期:2017-11-10
常青, 张保卫, 金宏. 2003. 从12s RNA基因序列推测鹭类13种鸟类的系统发生关系[J]. 动物学报, 49(2):205-210.
高英凯, 苗正旺, 苏小茜, 等. 2009. 74种鸟类线粒体基因组碱基组成及特征分析[J]. 云南农业大学学报, 24(1):51-58.
韩联宪, 何臣相, 王斌, 等. 2015. 云南发现白腹鹭[J]. 四川动物, 34(2):281.
黄原. 2012. 分子系统发生学[M]. 北京:科学出版社.
蒋志刚, 江建平, 王跃招, 等. 2016. 中国脊椎动物红色名录[J]. 生物多样性, 24(5):500-551.
张保卫, 常青, 魏辅文. 2002. 鹭科鸟类分类及系统学研究进展[J]. 动物学杂志, 37(3):84-88.
张国萍, 王蔚, 朱世杰, 等. 2005. 鹳形目12种鸟类核c-mos基因和线粒体12s rRNA基因序列分析及其系统发生研究[J]. 四川动物, 24(4):500-506.
郑光美. 2017. 中国鸟类分类与分布名录(第三版)[M]. 北京:科学出版社.
郑作新. 1964. 中国鸟类系统检索[M]. 北京:科学出版社:9-12.
郑作新. 1994. 中国鸟类种和亚种分类名录大全[M]. 北京:科学出版社:9-11.
钟东, 赵贵军, 张振书, 等. 2002. 基因组内碱基分布整体均衡与局部不均衡的研究进展[J]. 遗传, 24(3):351-355.
Arnaiz-Villena A, Guillén J, Ruiz-del-Valle V, et al. 2001. Phylogeography of crossbills, bullfinches, grosbeaks, and rosefinches[J]. Cellular and Molecular Life Sciences, 58(8):1159-1166.
Ben FK, Edward CD, Martin WW. 1975. A field guide to the birds of south-east Asia[M]. Boston:Houghton Mifflin Press:41-47.
Gill F, Donsker D. 2014. IOC world bird list (v 4.3)[EB/OL].[2017-11-07]. DOI:10.14344/IOC.ML.4.3.
Hebert PDN, Stoeckle MY, Zemlak TS, et al. 2004. Identification of birds through DNA barcodes[J]. PLoS Biology, 2(10):1657-1663.
Hermann H, Richard F, John P. 1972. The birds of Britain and Europe with north African and the middle East[M]. London:Collins Press:34-39.
Hoyo JD, Elliott A, Sargatal J. 1992. Handbook of the birds of the world vol. 5[M]. Spain:Lynx Edicions Press:407.
Huelsenbeck JP, Ronquist F. 2003. MrBayes:Bayesian inference of phylogenetic trees[J]. Bioinformatics, 17:754-755.
IUCN. 2016. The IUCN red list of threatened species[EB/OL]. (2016-10-01)[2017-11-07].
Kerr KCR, Stoeckle MY, Dove CJ, et al. 2007. Comprehensive DNA barcode coverage of north American birds[J]. Molecular Ecology Notes, 7(4):535-543.
Mindell DP. 1997. Avian molecular evolution and systematics[M]. San Diego:Academic Press:84-86.Peters JL. 1945. Checklist of birds of the world vol. 5[M]. Cambridge, MA:Harvard University Press.
Rasmussen PC, Anderton JC. 2005. Birds of south Asia:the ripley guide[M]. Washington, Barcelona:Smithsonian Institution and Lynx Edicions.
Saetre GP, Borge T, Lindell J, et al. 2001. Speciation, introgressive hybridization and nonlinear rate of molecular evolution in flycatchers[J]. Molecular Ecology, 10(3):737-749.
Sibley CG, Monoroe BLJ. 1990. Distribution and taxonomy of birds of the world[M]. New Haven & London:Yale University Press:302-310.
Thompson JD, Gibson TJ, Plewniak F, et al. 1997. The Clustal X windows interface:flexible strategies for multiple sequence alignment aided by quality analysis tools[J]. Nucleic Acids Resreach, 24:4876-4882.
Webb DM, Moore WS. 2005. A phylogenetic analysis of woodpeckers and their allies using 12S, Cyt b and COⅠ nucleotide sequences (Class Aves; Order Piciformes)[J]. Molecular Phylogenetics Evolution, 36:233-248.
Weibel AC, Moore WS. 2002. Molecular phylogeny of a cosmopolitan group of woodpeckers (Genus Picoides) based on COⅠ and Cyt b mitochondrial gene sequences[J]. Molecular Phylogenetics Evolution, 22:65-75.
CopyRight©2019 Editorial Office of Sichuan Journal of Zoology