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Issue:ISSN 1000-7083
          CN 51-1193/Q
Director:Sichuan Association for Science and Technology
Sponsored by:Sichuan Society of Zoologists; Chengdu Giant Panda Breeding Research Foundation; Sichuan Association of Wildlife Conservation; Sichuan University
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Your Position :Home->Past Journals Catalog->2018 Vol.37 No.3

Population Genetic Structure and Geographic Differentiation of Saurogobio punctatus(Teleostei, Cypriniformes, Cyprinidae) in the Yangtze River Basin Based on Cytochrome b Gene Sequences
Author of the article:LI Xiaobing1,2, TANG Qiongying1, YU Dan1, LIU Huanzhang1*
Author's Workplace:1. The Key Laboratory of Aquatic Biodiversity and Conservation, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China;
2. University of the Chinese Academy of Sciences, Beijing 100049, China
Key Words:Saurogobio punctatus; Yangtze River basin; Cyt b; genetic structure; geographic differentiation
Abstract:The population genetic structure and geographic differentiation process of 3 Saurogobio punctatus populations from the Chishui River (one branch of the upper Yangtze River), the upper Yangtze River main stream, and the middle Yangtze River were studied based on mtDNA cytochrome b gene sequences of 303 individuals. The analyzed cytochrome b gene sequences were 1 097 bp in length, with 80 variable sites including 34 parsimony informative sites. A total of 49 haplotypes were identified from 303 individuals, which showed high haplotype diversity (Hd=0.803) and low nucleotide diversity (Pi=0.003 71), respectively. Based on haplotype dataset, phylogenetic trees which were constructed using neighbor-joining, maximum likelihood and Bayesian inference methods showed that all haplotypes from the middle Yangtze River clustered together and formed a monophyletic group. This group locates in the derived position of the phylogenetic trees, whereas haplotypes from the upper Yangtze River main stream and the Chishui River failed to form a monophyletic group with relative basal positions in the phylogenetic tree. Extensive gene flow might exist between populations of the upper Yangtze River main stream and Chishui River since many haplotypes were shared by them with a low genetic differentiation index (FST=0.029 4), indicating a close relationship. The FST values between the middle Yangtze River and Chishui River populations, and between the middle Yangtze River and the upper Yangtze River main stream populations were 0.614 0 and 0.706 0, respectively, indicating a high level differentiation. Neutral test and mismatch analysis showed that both the populations of upper Yangtze River main stream and Chishui River had experienced population expansion, but not in the middle Yangtze River population. Bayesian skyline plot (BSP) analysis showed that S. punctatus populations started the expansion from 0.20 Ma BP, and lasted until the end of the last interglacial period, the Marine Isotope Stage 5, then experienced a rapid expansion. According to BSP analysis and haplotype network, we inferred that the origination center of S. punctatus population may be the upper Yangtze River, and then gradually spread to the middle Yangtze River via population expansion, and eventually evolved into a distinct genetic population.
2018,37(3): 251-259 收稿日期:2015-11-25
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