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Issue:ISSN 1000-7083
          CN 51-1193/Q
Director:Sichuan Association for Science and Technology
Sponsored by:Sichuan Society of Zoologists; Chengdu Giant Panda Breeding Research Foundation; Sichuan Association of Wildlife Conservation; Sichuan University
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Your Position :Home->Past Journals Catalog->2018 Vol.37 No.2

Establishment of Metastasis Lung Cancer Model by Injection of EMT-6 Cells in BALB/c Mice
Author of the article:WEI Shihang1, PENG Xu2, WEI Shiyuan1, SHI Xueni1, HE Xueling2, LIU Yan2, YIN Hailin2*
Author's Workplace:1. Key Laboratory of Bio-Resources and Eco-Environment of Ministry of Education, College of Life Sciences, Sichuan University, Chengdu 610065, China;
2. Laboratory Animal Centre, Sichuan University, Chengdu 610041, China
Key Words:EMT-6 cell; animal model of metastasis lung cancer; BALB/c mouse
Abstract:Objective To establish a metastasis lung cancer model with apparent clinical, stable and repeatable characteristics in BALB/c mice by intravenous injection of EMT-6 breast-tumor cells. Methods Cells of EMT-6 murine mammary carcinoma at logarithmic growth phase were harvested by 0.25% trypsin digestion, and equilibrated at 3 densities of1×106 cells per mL (high concentration), 5×105 cells per mL (medium concentration), and 1×105 cells per mL (low concentration) in phosphate-buffered saline. And then 0.2 mL EMT-6 tumor cells were intravenously injected. The evaluation indicators and biological characteristics such as clinic appearance, the time of tumor formation, survival time, the tumor growth and pathological characteristics of metastasis lung models with different concentrations were then studied to screen the best metastasis lung cancer model. Finally, the repeatability and stability of the model were determined by 3 repeated experiments to successfully establish the metastasis lung cancer model in BALB/c mice. Results BALB/c mice were anatomized after EMT-6 tumor cells injection, and the results showed that in the group of high concentration treatment, tumor nodes on lung surface began to appear at day 7 and all animals died in 18 d; in the group of medium concentration, tumor nodes on[LM] lung surface began to appear at day 7 and all animals died in 28 d; in the group of low concentration, tumor nodes on lung surface appeared partially at day 14, all mice lung surface had tumors at day 21, the number of tumors reach the highest level (an average of 12-15/mouse), the volume of tumors became much bigger at day 35, mice began to die at day 24, and all mice died in 42 d. Conclusion We concluded that the establishment of mice models using low EMT-6 cell concentration can provide suitable 4-week time window for treatment, observation and test from tumor formation to dead, the histopathology of models' lung was consistent with clinic features of lung cancer, the clinic appearance was easy to identify, and the repeatability and stability tests showed the models were consistent among the 3 times.
2018,37(2): 191-196 收稿日期:2017-06-06
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