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Issue:ISSN 1000-7083
          CN 51-1193/Q
Director:Sichuan Association for Science and Technology
Sponsored by:Sichuan Society of Zoologists; Chengdu Giant Panda Breeding Research Foundation; Sichuan Association of Wildlife Conservation; Sichuan University
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Your Position :Home->Past Journals Catalog->2018 Vol.37 No.2

Enhancement of Immune Responses to PCV-2 Vaccine by Porcine IL-2 and Fusion IL-4/6 Gene Entrapped in Chitosan Nanoparticles
Author of the article:CHEN Yi1#, SONG Tingyu1#, LI Jinhai2#, XIAO Yongle1, ZENG Guangzhi2, WAN Xiaoping1, YANG Luyi1, FANG Pengfei2*, WANG Zezhou3*, GAO Rong1*
Author's Workplace:1. Key Laboratory of Bio-Resource and Eco-Environment of Ministry of Education, Key Laboratory for Animal Disease Prevention and Food Safety of Sichuan Province, College of Life Sciences, Sichuan University, Chengdu 610065, China;
2. Sichuan Huapai Biopharmaceutical Company, Chengdu 610026, China;
3. Center for Animal Disease Control of Sichuan Province, Chengdu 610035, China
Key Words:piglet; gene co-expression; IL-2; fusion IL-4/6; immunity; PCV-2 vaccine
Abstract:Objective In order to develop a novel effective immunomodulator to enhance pig resistance against post-weaning multisystemic wasting syndrome, we used the recombinant plasmid co-expressing the pig interleukin-2 (IL-2) and fusion interleukin-4/6 (IL-4/6) proteins which we constructed before, and studied its effect on immune response of piglets. Methods The recombinant plasmid was first encapsulated in chitosan nanoparticles by the ionotropic gelation method, and generated VRIL-4/6-2-CS. Then piglets were divided into 2 groups and intramuscularly injected with VRIL-4/6-2-CS or saline followed by the injection of pig circovirus-2 (PCV-2) vaccine, respectively. The blood was collected from each piglet on days 0, 7, 14 and 28 to assay the immunological changes. Result The number of IgG2a, CD4+, CD8+ T cells increased significantly in the sera or blood of piglets that treated with VRIL-4/6-2-CS (P<0.05). Furthermore, the expression levels of IL-2, IL-4, IL-6, TNF-α, TLRs (TLR-2, 7) and STATs (STAT-1, 2, 3) genes elevated significantly in the piglets that treated with VRIL-4/6-2-CS (P<0.05). Although no significant differences were observed in the levels of PCV-2-specific antibody, the growth performance of VRIL-4/6-2-CS treated piglets was remarkably improved in comparison with that of control (P<0.05). Conclusion VRIL-4/6-2 entrapped in chitosan is a promising effective adjuvant to promote the immune responses of pig vaccinated with PCV-2.
2018,37(2): 156-163 收稿日期:2017-03-30
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