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Issue:ISSN 1000-7083
          CN 51-1193/Q
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Sponsored by:Sichuan Society of Zoologists; Chengdu Giant Panda Breeding Research Foundation; Sichuan Association of Wildlife Conservation; Sichuan University
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Your Position :Home->Past Journals Catalog->2018 Vol.37 No.2

DNA Barcoding of Bambusa emeiensis-Feeding Leafhoppers in China for Species Identification
Author of the article:YAO Yalin1,2,3,4, LUO Qiang2,3,4, DONG Mengshu2,3,4, CHEN Xiangsheng2,3,4, YANG Lin2,3,4*
Author's Workplace:1. College of Forestry, Guizhou University, Guiyang 550025, China;
2. Special Key Laboratory for Development and Utilization of Insect Resources of Guizhou University, Guiyang 550025, China;
3. Institute of Entomology, Guizhou University, Guiyang 550025, China;
4. Key Laboratory for Plant Pests Management of Mountainous Region in Guizhou Province, Guiyang 550025, China
Key Words:DNA barcoding; COⅠ; 16S rRNA; species identification; leafhoppers; Bambusa emeiensis
Abstract:Leafhoppers play a key role in biodiversity and plant protection of agricultural and forestry ecosystems. However, species identification of leafhoppers is a challenge for this subject due to their morphology diversity. DNA barcoding provides an efficient method for species identification. In the present study, partial COⅠ and 16S rRNA gene sequences from 12 Bambusa emeiesis-feeding leafhopper species in China were amplified. A neighbor-joining phylogenetic tree was constructed based on Kimura 2-parameter (K2P) genetic distance, and further studied using Klee-diagram vector analysis. Intra-specific K2P genetic distance averaged 0.004 for COⅠ, and 0.003 for 16S rRNA; inter-specific K2P genetic distance averaged 0.283 and 0.257 for COⅠ and 16S rRNA, respectively. An obvious ‘barcoding gap’ was detected for all species in both markers, and all individuals were clustered together in both phylogeny trees constructed using COⅠ and 16S rRNA. The result of cluster analyses based on COⅠ and 16S rRNA gene sequences were basically consistent with that of morphological identification, and different individuals tested in this study were gathered in a common branch of the dendrograms. Moreover, the result of Klee-diagram vector analysis was also consistent with phylogeny. Hence, both COⅠ and 16S rRNA genes appear to be accurate and efficient markers for bamboo leafhoppers identification in China.
2018,37(2): 127-138 收稿日期:2017-09-23
Del-Prado R, Cubas P, Lumbsch HT, et al. 2010. Genetic distances within and among species in monophyletic lineages of Parmeliceae (Ascomycota) as a tool for taxon delimitation[J]. Molecular Phylogenetics and Evolution, 56:125-133.
Dietrich CH, Whitcomb RF, Black IV WC. 1997. Phylogeny of the grassland leafhopper genus Flexamia (Homoptera:Cicadellidae) based on mitochondrial DNA sequences[J]. Molecular Phylogenetics and Evolution, 8:139-149.
Fang QQ, Black IV WC, Blocker HD, et al. 1993. A phylogeny of New World Deltocephalus-like leafhopper genera based on mitochondrial 16S ribosomal DNA sequences[J]. Molecular Phylogenetics and Evolution, 2(2):119-131.
Folmer O, Black M, Hoeh W, et al. 1994. DNA primers for amplification of mitochondrial cytochrome c oxidase subunit Ⅰ from diverse metazoan invertebrates[J]. Molecular Marine Biology and Biotechnology, 3(5):294.
Hajibabaei M, Janzen DH, Burns JM, et al. 2006. DNA barcodes distinguish species of tropical Lepidoptera[J]. Proceedings of the National Academy of Sciences of the United States of America, 103:968-971.
Hebert PDN, Penton EH, Burns JM, et al. 2004a. Ten species in one:DNA barcoding reveals cryptic species in the neotropical skipper butterfly Astraptes fulgerator[J]. Proceedings of the National Academy of Sciences of the United States of America, 101:14812-14817.
Hebert PDN, Ratnasingham S, Waard JR. 2003. Barcoding animal life:cytochrome c oxidase subunit Ⅰ divergences among closely related species[J]. Proceedings of the Royal Society B-Biological Sciences, 270:S96-S99.
Hebert PDN, Stoeckle MY, Zemlak TS, et al. 2004b. Identification of birds through DNA barcodes[J]. PLoS Biology,2:1657-1663.
Köhler F. 2007. From DNA taxonomy to barcoding:how a vague idea evolved into a biosystematic tool[J]. Zoosystematics and Evolution, 83:44-51.
Miglietta MP, Schuchert P, Cunningham CW. 2009. Reconciling genealogical and morphological species in a worldwide study of the family Hydractiniidae (Cnidaria, Hydrozoa)[J]. Zoologica Scripta, 38:403-430.
Miranda LS, Collins AG, Marques AC. 2010. Molecules clarify a Cnidarian life cycle-the "Hydrozoan" Microhydrula limopsicola is an early life stage of the Staurozoan Haliclystus antarcticus[J]. PLoS ONE, 5:e10182. DOI:10.1371/journal.pone.0010182.
Moura CJ, Cunha MR, Porteiro FM, et al. 2011a. Polyphyly and cryptic diversity in the hydrozoan families Lafoeidae and Hebellidae (Cnidaria:Hydrozoa)[J]. Invertebrate Systematics, 25:454-470.
Moura CJ, Cunha MR, Porteiro FM, et al. 2011b. The use of the DNA barcode gene 16S mRNA for the clarification of taxonomic problems within the family Sertulariidae (Cnidaria, Hydrozoa)[J]. Zoologica Scripta, 40:520-537.
Ortman BD, Bucklin A, Pagès F, et al. 2010. DNA barcoding the Medusozoa using mtCOⅠ[J]. Deep Sea Research Part Ⅱ:Topical Studies in Oceanography, 57(24-26):2148-2156.
Ratnasingham S, Hebert PDN. 2007. BOLD:the barcode of life data system ([J]. Molecular Ecology Notes, 7:355-364.
Schuchert P. 2006. The European athecate hydroids and their medusae (Hydrozoa, Cnidaria):Capitata Part 1[J]. Revue Suisse de Zoologie, 113:325-410.
Sinniger F, Reimer JD, Pawlowski J. 2008. Potential of DNA sequences to identify zoanthids (Cnidaria:Zoantharia)[J]. Zoological Science, 25:1253-1260.
Sirovich L, Stoeckle MY, Zhang Y. 2009. A scalable method for analysis and display of DNA sequences[J]. PLoS ONE, 4:e7051. DOI:10.1371/journal.pone.0007051.
Sirovich L, Stoeckle MY, Zhang Y. 2010. Structural analysis of biodiversity[J]. PLoS ONE, 5:e9266. DOI:10.1371/journal.pone.0009266.
Stampar SN, Maronna MM, Vermeij MJA, et al. 2012. Evolutionary diversification of banded tube-dwelling anemones (Cnidaria; Ceriantharia; Isarachnanthus) in the Atlantic Ocean[J]. PLoS ONE, 7:e41091. DOI:10.1371/journal.pone.0041091.
Stoeckle MY, Coffran C. 2013. TreeParser-aided Klee diagrams display taxonomic clusters in DNA barcode and nuclear gene datasets[J]. Scientific Reports, 3:2635-2637.
Sun W, Zhang ZT, Dong H, et al. 2013. Analysis of genetic differentiation and gene flow among different geographic populations of Oedaleus infernalis (Orthoptera:Acrididae) based on mtDNA COⅠ gene sequences[J]. Acta Entomologica Sinica, 56(8):907-916.
Sun Y, Li Q, Kong L, et al. 2012. DNA barcoding of Caenogastropoda along coast of China based on the COⅠ gene[J]. Molecular Ecology Resources, 12:209-218.
Ward RD, Zemlak TS, Innes BH, et al. 2005. DNA barcoding Australia's fish species[J]. Philosophical Transactions:Biological Sciences, 360:1847-1857.
Yang L, Chen XS. 2011. Review of bamboo-feeding leafhopper genus Mukaria Distant (Hemiptera:Cicadellidae:Mukariinae) with description of a new species from China[J]. Zootaxa, 2882:27-34.
Yang ZH, Rannala B. 2010. Bayesian species delimitation using multilocus sequence data[J]. Proceedings of the National Academy of Sciences of the United States of America, 107:9264-9269.
Zemlak TS, Ward RD, Connell AD, et al. 2009. DNA barcoding reveals overlooked marine fishes[J]. Molecular Ecology Resources, 9:237-242.
Zhang JZ, Guo YP, Ma EB. 2004. DNA extraction and RAPD analysis of grasshopper samples kept under different conditions[J]. Chinese Journal of Zoology, 39(2):53-57.
Zhang MZ, Kang L. 2001. Extraction of total DNA from locusts and optimization of reaction conditions for RAPD analysis[J]. Zoological Research, 22(1):20-26.
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