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Issue:ISSN 1000-7083
          CN 51-1193/Q
Director:Sichuan Association for Science and Technology
Sponsored by:Sichuan Society of Zoologists; Chengdu Giant Panda Breeding Research Foundation; Sichuan Association of Wildlife Conservation; Sichuan University
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Your Position :Home->Past Journals Catalog->2018 Vol.37 No.2

Whole Genome Sequencing and Analysis of Medicinal Periplaneta americana
Author of the article:JIN Jiazheng1, LI Wujiao2, MOU Biqin3, SHEN Yongmei3, GENG Funeng2, YUE Bisong1, FAN Zhenxin1,3*
Author's Workplace:1. Key Laboratory of Bio-Resources and Eco-Environment of Ministry of Education, College of Life Sciences, Sichuan University, Chengdu 610065, China;
2. Sichuan Key Laboratory of Medicinal American Cockroach, Xichang, Sichuan Province 615000, China;
3. Sichuan Medicinal Animals Engineering Research Center, Xichang, Sichuan Province 615000, China
Key Words:Periplaneta americana; whole genome sequencing; genome assembly; genome annotation
Abstract:In this study, the whole genomic DNA of medicinal American cockroach (Periplaneta americana) was firstly sequenced by Illumina HiSeq 2000 and PacBio SMRT platforms. In total, 1.4 Tb Illumina data and 33.81 Gb PacBio data were retained after the quality control of the raw sequence data. After genome assembly using these clean data, here we confirm that the complete genome size of P. americana is 3.26 Gb, and this is only smaller than Locusta migratoria among all of the reported genomes of insects. Specifically, the heterozygosity rate of P. americana genome is 0.635%, and 62.38% of the genome sequences are found to be repeats sequences. The lengths of Contig N50 and scaffold N50 are 28.2 kb and 315 kb, respectively. Moreover, 88.1% of the single copy orthologs are successfully detected by using BUSCO analysis, and the results of gene prediction based on De novo, homology and transcriptome showed that there are 14 568 genes in the genome of P. americana, and 92.4% of them can be functionally annotated. Our study provides the first whole genome sequence of P. americana, and also the first genomic information of the insect in Periplaneta genus, which can significantly contribute to further genome based analysis and the exploration of medicinal resources.
2018,37(2): 121-126 收稿日期:2017-11-28
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