Latest Cover

Online Office

Contact Us

Issue:ISSN 1000-7083
          CN 51-1193/Q
Director:Sichuan Association for Science and Technology
Sponsored by:Sichuan Society of Zoologists; Chengdu Giant Panda Breeding Research Foundation; Sichuan Association of Wildlife Conservation; Sichuan University
Address:College of Life Sciences, Sichuan University, No.29, Wangjiang Road, Chengdu, Sichuan Province, 610064, China
Fax:+86-28-85410485 &
Your Position :Home->Past Journals Catalog->2018 Vol.37 No.1

Nucleus Accumbens Microinjection Jak/Stat3 Inhibitor Regulate Energy Metabolism of Lasiopodomys brandtii
Author of the article:YAN Lixin1, ZHANG Zhibin2, SONG Mingjing1*
Author's Workplace:1. Key Laboratory of Human Diseases Comparative Medicine, Ministry of Health, Institute of Laboratory Animal Science, Chinese Academy of Medical Sciences & Comparative Medicine Center, Peking Union Medical College, Beijing 100021, China;
2. Institute of Zoology, Chinese Academy of Science, Beijing 100101, China
Key Words:nucleus accumbens; leptin; Lasiopodomys brandtii; energy metabolism; body mass
Abstract:Objective To explore the effect of impaired leptin-Stat3 in nucleus accumbens on the metabolism and body mass of Lasiopodomys brandtii. Methods Inhibitor of Jak/Stat3 (WP1066) was injected into the nucleus accumbens of L. brandtii, and then the basic metabolism was tested by TSE metabolic cage system followed by monitoring the body mass.Results Jak/Stat3 could significantly decrease the levels of P-Stat3 and leptin in nucleus accumbens (P<0.05). However, there was no significant difference in the levels of serum leptin and blood glucose (P>0.05). The result of TSE metabolic cage system showed that the energy expenditure, cumulative O2 consumption and cumulative locomotor activity decreased significantly, while the respiratory exchange rate and cumulative drink increased significantly in experimental group (P<0.05). Moreover, the average body mass of experimental group increased significantly (P<0.05). Conclusion Inhibition of leptin-Stat3 pathway in nucleus accumbens of L. brandtii can influence energy metabolism and body mass of L. brandtii in 10 days.
2018,37(1): 57-61 收稿日期:2017-08-24
基金项目:国家自然科学基金项目(31471790);农业虫害鼠害综合治理研究国家重点实验室开放研究基金项目(Chinese IPM1615)
Banks WA. 2001. Enhanced leptin transport across the blood-brain barrier by alpha 1-adrenergic agents[J]. Brain Research, 899(1-2):209-217.
Bouret SG, Draper SJ, Simerly RB. 2004. Trophic action of leptin on hypothalamic neurons that regulate feeding[J]. Science, 304(5667):108-110.
Chekhranova MK, Karpova SK, Iatsyshina SB, et al. 2008. A new mutation c.422C>G (p.S141C) in homo-and heterozygous forms of the human leptin gene[J]. Bioorganicheskaia Khimiia, 34(6):854-856.
Fatima W, Shahid A, Imran M, et al. 2011. Leptin deficiency and leptin gene mutations in obese children from Pakistan[J]. International Journal of Pediatric Obesity, 6(5-6):419-427.
Fischer-Posovszky P, von Schnurbein J, Moepps B, et al. 2010. A new missense mutation in the leptin gene causes mild obesity and hypogonadism without affecting T cell responsiveness[J]. The Journal of Clinical Endocrinology & Metabolism, 95(6):2836-2840.
Hainer V, Kunesova M, Parizkova J, et al. 2000. Respiratory quotient in obesity:its association with an ability to retain weight loss and with parental obesity[J]. Sborník Lékarsky, 101(1):99-104.
Laing BT, Do K, Matsubara T, et al. 2016. Voluntary exercise improves hypothalamic and metabolic function in obese mice[J]. Journal of Endocrinology, 229(2):109-122.
Liu XY, Yang DB, Xu YC, et al. 2016. Photoperiod induced obesity in the Brandt's vole (Lasiopodomys brandtii):a model of healthy obesity?[J]. Disease Models & Mechanisms, 9(11):1357-1366.
Lou MF, Shen W, Fu RS, et al. 2015. Maternal dietary protein supplement confers long-term sex-specific beneficial consequences of obesity resistance and glucose tolerance to the offspring in Brandt's voles[J]. Comparative Biochemistry and Physiology Part A:Molecular & Integrative Physiology, 182:38-44.
Marra M, Scalfi L, Contaldo F, et al. 2004. Fasting respiratory quotient as a predictor of long-term weight changes in non-obese women[J]. Annals Nutrition Metabolism, 48(3):189-192.
Mazen I, El-Gammal M, Abdel-Hamid M, et al. 2009. A novel homozygous missense mutation of the leptin gene (N103K) in an obese Egyptian patient[J]. Molecular Genetics Metabolism, 97(4):305-308.
Patterson CM, Bouret SG, Dunn-Meynell A, et al. 2009. Three weeks of postweaning exercise in DIO rats produces prolonged increases in central leptin sensitivity and signaling[J]. American Journal of Physiology Regulatory Integrative Comparative Physiology, 296(3):R537-R548.
Schutz Y. 1995. Abnormalities of fuel utilization as predisposing to the development of obesity in humans[J]. Obesity, 3(S2):173S-178S.
Strobel A, Issad T, Camoin L, et al. 1998. A leptin missense mutation associated with hypogonadism and morbid obesity[J]. Nature Genetics, 18(3):213-215.
Thakur S, Kumar A, Dubey S, et al. 2014. A novel mutation of the leptin gene in an Indian patient[J]. Clinal Genetics, 86(4):391-393.
Wabitsch M, Funcke JB, Lennerz B, et al. 2015. Biologically inactive leptin and early-onset extreme obesity[J]. The New England Journal of Medicine, 372(1):48-54.
Zhang XY, Lou MF, Shen W, et al. 2017. A maternal low-fiber diet predisposes offspring to improved metabolic phenotypes in adulthood in an herbivorous rodent[J]. Physiological Biochemical Zoology, 90(1):75-84.
CopyRight©2018 Editorial Office of Sichuan Journal of Zoology