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Issue:ISSN 1000-7083
          CN 51-1193/Q
Director:Sichuan Association for Science and Technology
Sponsored by:Sichuan Society of Zoologists; Chengdu Giant Panda Breeding Research Foundation; Sichuan Association of Wildlife Conservation; Sichuan University
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Your Position :Home->Past Journals Catalog->2018 Vol.37 No.1

Effect of Cannabinoid Receptor 1 on Lipid Metabolism in Diet-induced Obese Mice
Author of the article:WEI Liwen1, YUAN Zhangqin2, ZHAO Mingde1, HAN Jianhong1, GU Congwei1, FU Lu1*
Author's Workplace:1. Department of Laboratory Animal, Southwest Medical University, Luzhou, Sichuan Province 646000, China;
2. Department of Orthopaedics, The First Affiliated Hospital, Orthopaedic Institute, Soochow University, Suzhou, Jiangsu Province 215006, China
Key Words:cannabinoid receptor 1; rimonabant; carnitine palmitoyltransferase-1; obesity; lipid metabolism
Abstract:Objective To study the regulatory mechanism of cannabinoid receptor 1 (CB1) on the lipid metabolism of diet-induced obese mice.Methods The C57BL/6J male mice were feed with high-fat diet, and then the effect of rimonabant on the body mass, liver mass and serum biochemical index of mice were detected. In particular, the mRNA levels ofCB1 and carnitine palmitoyltransterase-1(CPT1), and protein levels of CB1 in subcutaneous fat, visceral fat, skeletal muscle, liver and heart were also tested. Results It was showed the body mass and liver mass, as well as the mRNA and protein levels of CB1 were all significantly decreased in rimonabant-treated mice (P<0.05). Simultaneously, the levels of total cholesterol, high density lipoprotein, adiponectin and leptin were improved (P<0.05). Furthermore, the mRNA levels of carnitine palmitoyltransterase-1A (CPT1A) and carnitine palmitoyltransterase-1B (CPT1B) in each tissue were increased, while no obviously difference was detected in the expression ofCPT1A andCPT1B in mouse heart. Conclusion It was suggested that CB1 can regulate lipid metabolism by controlling the expression ofCPT1A andCPT1B genes. A basis for clinical treatment of lipid metabolism disorders was provided.
2018,37(1): 51-56 收稿日期:2017-05-22
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