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Issue:ISSN 1000-7083
          CN 51-1193/Q
Director:Sichuan Association for Science and Technology
Sponsored by:Sichuan Society of Zoologists; Chengdu Giant Panda Breeding Research Foundation; Sichuan Association of Wildlife Conservation; Sichuan University
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Your Position :Home->Past Journals Catalog->2018 Vol.37 No.1

cDNA Cloning, Characterization and Expression Analysis of Peroxiredoxin Gene in Carassius auratus gibelio
Author of the article:SHUI Dianzhang#, CHEN Shengfeng#, WU Ping*, YE Yuantu, CHEN Jia, ZHANG Jingjing
Author's Workplace:School of Biology & Basic Medical Sciences, Soochow University, Suzhou, Jiangsu Province 215123, China
Key Words:Carassius auratus gibelio; peroxiredoxin; cloning; sequence analysis; tissue distribution
Abstract:In this study, the full-length cDNA sequence of peroxiredoxin (Prx) gene was cloned from the liver of Carassius auratus gibelio by reverse transcription-polymerase chain reaction and rapid amplification of cDNA ends methods. The structure and speculated function of Prx gene were analyzed. The full-length of CaPrx was comprised of 1 035 nucleotides with a 3'untranslated region (UTR) of 366 bp, 5'UTR of 75 bp and an open reading frame of 594 bp, encoding 197 amino acids. The predicted molecular mass and estimated isoelectric point were 21.83 kD and 5.93. In addition, there was a conservative Cys in the N-terminal and C-terminal, respectively, and this suggested that the CaPrx is belonging to 2-Cys Prxs family. Sequence comparison revealed that the deduced amino acid sequence of CaPrx was highly homologous to the Prx homologs of Mylopharyngodon piceus (98%) and Rattus norvegicus (81%). Furthermore, quantitative real-time PCR analysis showed that CaPrx could be detected in various tissues. The highest expression level was detected in hemocyte and liver followed by hemocyte, and little expression of this gene was detected in head kidney, gills and brain. Compared with the diseased fish, the levels of CaPrx was decreased significantly in liver, which indicated that the CaPrx might be involved in immune response against cyprinid herpesvirus Ⅱ infection in C. auratus gibelio.
2018,37(1): 35-44 收稿日期:2017-07-27
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