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Issue:ISSN 1000-7083
          CN 51-1193/Q
Director:Sichuan Association for Science and Technology
Sponsored by:Sichuan Society of Zoologists; Chengdu Giant Panda Breeding Research Foundation; Sichuan Association of Wildlife Conservation; Sichuan University
Address:College of Life Sciences, Sichuan University, No.29, Wangjiang Road, Chengdu, Sichuan Province, 610064, China
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Your Position :Home->Past Journals Catalog->2017 Vol.36 No.6

Studies on the Micro-ultrastructure of Micropterus salmoides Sperm
Author of the article:QIAO Zhigang, ZHANG Xiaoguang, SHEN Fangfang, LIU Shuyan, JIA Wu
Author's Workplace:Fisheries College, Henan Normal University, Xinxiang, Henan Province 453007, China
Key Words:Micropterus salmoides; sperm; ultrastructure
Abstract:The current study investigated the ultrastructure of largemouth bass (Micropterus salmoides) sperm using scanning electron microscope and transmission electron microscope. The sperm of M. salmoides was composed of three major parts:a nearly spherical head without an acrosome, a unconspicuous midpiece, and a slender flagellum. The full-length of sperm is 25.07 μm±4.93 μm (n=30) with a spherical head length of 1.73 μm±0.29 μm in diameter (n=30) and a slender flagellum length of 23.00 μm±4.86 μm long (n=30). The head of sperm is mainly composed of a mushroom-shaped nuclei, and the clustering electron-dense is separated by an electronlucent area. The nucleus near the flagellum segment is a depression, and it forms shallow nuclear fossa. The midpiece of sperm contains the centriolar complex and the sleeve. The centriolar complex is composed of the proximal and distal centriole. The proximal centriole is located inside the nuclear fossa and parallel to the cross axis of nuclei. The ‘T’ shape distal centriole locates outside the nuclear fossa and inside the sleeve is the base of flagellum, and it is perpendicular to the proximal centriole. There are 17±4 unequally sized mitochondria (n=30) inside the central space of both sides of sleeve. The flagellum of sperm stretched out of the central space of sleeve is mainly composed of the axoneme and the lateral fin. The axoneme comprised microtubule configurations in a ‘9+2’ microtubule pattern. The lateral fins are distributed on both sides of flagella. These results revealed that the sperm of M. salmoides should be categorized as Type I, which is clearly distinct from the sperm of other species from order Perciformes in the shape of sleeve, the number and shape of mitochondria. The sleeve structure of M. salmoides is accord with the typical sleeve structure of teleost sperm, but the shape and the number of mitochondria of M. salmoides are similar with amphibian species, indicating that M. salmoides is more original in phylogenetic development than other species of Perciformes.
2017,36(6): 686-691 收稿日期:2017-06-27
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