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Issue:ISSN 1000-7083
          CN 51-1193/Q
Director:Sichuan Association for Science and Technology
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Your Position :Home->Past Journals Catalog->2017 Vol.36 No.6

Molecular Portrait of Periplaneta americana Extracts on Colon Cancer Cells Based on RNA-seq Analysis
Author of the article:CHEN Feng1,2,3, CHEN Jiasong1,2,3, PENG Rui1,2,3, GENG Funeng3*, ZOU Fangdong1,2,3*
Author's Workplace:1. College of Life Sciences, Sichuan University, Chengdu 610065, China;
2. Sichuan Engineering Research Center for Medicinal Animals, Chengdu 610065, China;
3. Sichuan Key Laboratory for Medicinal American Cockroach, Chengdu 610065, China
Key Words:Periplaneta americana; extraction; transcriptome; differentially expressed genes; signaling pathways; functional annotation
Abstract:Periplaneta americana extracts can inhibit the growth of several tumors, and even induce tumor cell apoptosis. However, the influence of P. americana extracts on the signaling pathways in cancer cells is still unknown. In this study, RNA-seq method was used to investigate the changes of signaling pathways in colon cancer cells that treated with P. americana extracts and cisplatin. The results showed that, comparing to the untreated group, colon cancer cells that treated with 30 μM cisplatin and 0.6% KFXY had 1 901 differentially expressed genes (DEGs), including 1 555 up-regulated genes and 346 down-regulated; 30 μM cisplatin and 0.8% JFJJ treated group had 2 587 DEGs, including 2 183 up-regulated genes and 404 down-regulated; 30 μM cisplatin and 100 μg·mL-1 JF treated group had 1 488 DEGs, including 1 164 up-regulated genes and 324 down-regulated. GO analysis and KEGG pathway analysis showed that the up-regulated DEGs were enriched in p53 signaling pathway, cell adhesion and apoptosis, while the down-regulated DEGs were enriched in aminoacyl-tRNA biosynthesis, one carbon pool and biosynthesis of amino acids.
2017,36(6): 649-656 收稿日期:2017-03-31
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