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Issue:ISSN 1000-7083
          CN 51-1193/Q
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Your Position :Home->Past Journals Catalog->2017 Vol.36 No.6

Gene Cloning, Molecular Evolution and Tissue Expression of G Protein-coupled Receptor 161 in Chicken
Author of the article:ZHU Guoqiang, MO Chunheng, LI Zhengyang, WANG Yajun, LI Juan*
Author's Workplace:Key Laboratory of Bio-Resources and Eco-Environment of Ministry of Education, College of Life Sciences, Sichuan University, Chengdu 610065, China
Key Words:chicken; GPR161; gene cloning; molecular evolution; tissue expression
Abstract:G protein-coupled receptor 161 (GPR161) is a member of GPCRs, and this orphan receptor plays an important role in the lens development and neurulation of mammals. Recent study showed that the structure of GPR161 was similar with scaffolding proteins and thus may had a potential function in signal transduction. Using chicken as experimental model, the cDNA sequence, molecular evolution and tissue expression of chicken GPR161 gene were investigated in this study. The full-length coding sequence of chicken GPR161 gene was 1 566 bp in length and encoding 521 amino acids. Sequence analysis revealed that GPR161 of chicken had high sequence similarity with that of human (83.0%), mouse (82.6%) or zebrafish (65.8%). Molecular evolutionary genetical analysis showed that the relationship of GPR161 among chicken, human and mouse were closer than that of chicken and zebrafish. The result of real-time quantitative PCR showed that the mRNA level of GPR161 gene was abundant in testis or ovary, brain, heart and muscle. Our data provide a basis for the study of the physiological function of GPR161 gene in birds.
2017,36(6): 632-638 收稿日期:2017-05-02
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