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Issue:ISSN 1000-7083
          CN 51-1193/Q
Director:Sichuan Association for Science and Technology
Sponsored by:Sichuan Society of Zoologists; Chengdu Giant Panda Breeding Research Foundation; Sichuan Association of Wildlife Conservation; Sichuan University
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Your Position :Home->Past Journals Catalog->2017 Vol.36 No.6

The Role of the Sperm-specific Lactate Dehydrogenase in Ochotona curzoniae Tissues
Author of the article:WANG Zhijie, WANG Yang, AN Zhifang, WEI Linna, WEI Lian, WEI Dengbang*
Author's Workplace:College of Eco-environmental Engineering, Qinghai University, Xining 810016, China
Key Words:Ochotona curzoniae; tissues; Ldh-c gene; LDH enzymatic activity; lactic acid; ATP
Abstract:The plateau pika (Ochotona curzoniae) has a strong adaptability to a hypoxic plateau environment. Previous studies found that the sperm-specific lactate dehydrogenase (LDH-C4) gene is expressed in plateau pika somatic cell. In order to shed light on the role of LDH-C4 in plateau pika tissues, we silenced the Ldh-c gene in pika heart, liver and brain by RNA interference, and then the expression levels of Ldh-c gene in these tissues were determined by real-time PCR and Western Blot. Additionally, the activities of LDH, the contents of lactic acid and ATP were also measured by biochemical method. The results indicated that intraperitoneal injection of adenovirus pMultiRNAi-Ldhc could significantly reduce the expression of Ldh-c gene in tissues of plateau pika. Specifically, in the mRNA and protein levels, the expression levels of Ldh-c gene in heart were decreased by 48.11% and 19.27%; in liver were decreased by 70.16% and 25.82%; in brain were decreased by 49.08% and 25.36%, respectively. Meanwhile, when Ldh-c gene expression was silenced, the activities of LDH, the contents of lactic acid and ATP in each tested tissues were generally decreased by 25.58%, 41.94% and 21.23%; 28.16%, 15.90% and 24.66%; 16.65%, 12.78% and 18.50%, respectively. These results suggested that under the condition of severe hypoxia, the heart, liver and brain tissues of plateau pika can generate part of ATP uponLDH-C4 catalyzed anaerobic glycolysis and therefore enhance the adaptation to the hypoxic environments.
2017,36(6): 624-631 收稿日期:2017-05-08
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