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Issue:ISSN 1000-7083
          CN 51-1193/Q
Director:Sichuan Association for Science and Technology
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Your Position :Home->Past Journals Catalog->2017 Vol.36 No.5

Phylogenetic Relationship and Genetic Diversity of Calotes versicolor in Guangxi
Author of the article:WEI Xiaomei1, LIU Fan1, YAN Linmiao2, PAN Yajie3, ZHANG Yueyun1, ZHAO Chengjian1, XU Yongli1, LI Li1, HUANG Yong
Author's Workplace:1. Guangxi Botanical Garden of Medicinal Plants, Nanning 530023, China;
2. Guangxi Dongli Mechanic School, Nanning 530023, China;
3. Guangxi Teachers Education University, Nanning 530001, China
Key Words:Calotes; phylogeny; molecular evolution; population history dynamics; phylogeography
Abstract:Phylogenetic relationship of species is one of the most fundamental and important problem in evolutionary biology. In this study, we sequenced the ND2-tRNA mitochondrion gene of 90 Calotes versicolor, analyzed the haplotypes and the level of population differentiation (Fst), and constructed the phylogenetic relationship among haplotypes from different populations. Results showed that 45 haplotypes were identified from 90 individuals, which displayed relatively high haplotype diversity (0.941 8) and low nucleotide diversity (0.005 5), respectively. Six populations shared a common haplotype. Most populations had higher Fst values and significantly differentiated. The analysis of molecular variance (AMOVA) showed that overall population divergent variation was mainly from within populations. Phylogenetic trees based on haplotype dataset by using maximum parsimony, maximum likelihood method and bayesian inference methods showed that all haplotypes from geographical populations did not appear to be a distinct genetic clade, and the strict geographical distribution and differentiation, which may because of introgressive hybridization or incomplete lineage sorting. The result of network diagram analysis showed that all haplotypes had an indistinct genetic clade. Population history dynamic analysis showed that there was no expansion in each geographical population. However, mismatch analysis and bayesian skyline plot analysis indicated that overall C. versicolor populations experienced rapid expansion during 0.05 million years ago.
2017,36(5): 519-530 收稿日期:2017-03-03
DOI:10.11984/j.issn.1000-7083.20170067
分类号:Q959.6
基金项目:国家自然科学基金项目(31460559);广西自然科学基金项目(2014GXNSFBA118113)
作者简介:*通讯作者:黄勇,男,博士,从事两栖爬行动物系统、分子进化和动物地理学研究,E-mail:huangykiz@163.com
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