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Issue:ISSN 1000-7083
          CN 51-1193/Q
Director:Sichuan Association for Science and Technology
Sponsored by:Sichuan Society of Zoologists; Chengdu Giant Panda Breeding Research Foundation; Sichuan Association of Wildlife Conservation; Sichuan University
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Your Position :Home->Past Journals Catalog->2017 Vol.36 No.5

Effects of Heavy Metal Pollution on the Population Density and Biomass of Lumbricus rubellus from Weak Alkaline Farmlands
Author of the article:REN Liang, AI Shiwei, LIU Bailin, ZHANG Yingmei*
Author's Workplace:Gansu Key Laboratory of Biomonitoring and Bioremediation for Environmental Pollution, School of Life Sciences, Lanzhou University, Lanzhou 730000, China
Key Words:heavy metal pollution; Lumbricus rubellus; population density; biomass; antioxidase; metallothionein; neutral red retention time
Abstract:To investigate the toxic effect of heavy metal on the population density and biomass of Lumbricus rubellus from weak alkaline farmlands, in this study, the maize farmlands of Liangzhuang and Shuanghe villages in Baiyin city (Gansu province), which were mainly contaminated by Cd, Pb, Zn, and Cu for a long time, were selected as the study sites, and the maize farmlands of Youyuan village in Yongjing county which had similar natural conditions but with relatively unpolluted area was selected as the control site. The heavy metal contamination levels between the polluted and the control sites, the population density and biomass, antioxidant enzyme activities (SOD, CAT, GSH-PX), metallothionein (MT) concentration and lysosomal membrane damage degree of L. rubellus were comparatively studied. The results showed that there were no significant differences in the antioxidase activities of L. rubellus between the polluted and control sites, while extreme significantly higher MT concentration was found in the polluted sites (P<0.01), indicating that L. rubellus from the polluted sites might be involved in stress responses; the test of neutral red retention time showed an extremely significant shortening in the coelomic cells of L. rubellus from the polluted sites (P<0.01), this suggested that the lysosomal membrane was damaged; furthermore, the population density and biomass of L. rubellus were significantly (P<0.05) or extremely declined (P<0.01). According to the results of this study, we concluded that the significant decreased population density and biomass of L. rubellus from weakly alkaline sites might be related to the long-term heavy metal pollution which lead to the damage of lysosomal membrane, change of antioxidase activities and increased responses to the environmental stress.
2017,36(5): 507-512 收稿日期:2017-04-08
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