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Issue:ISSN 1000-7083
          CN 51-1193/Q
Director:Sichuan Association for Science and Technology
Sponsored by:Sichuan Society of Zoologists; Chengdu Giant Panda Breeding Research Foundation; Sichuan Association of Wildlife Conservation; Sichuan University
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Your Position :Home->Past Journals Catalog->2017 Vol.36 No.5

Molecular Cloning, Tissue Distribution and Expression of GnRH Gene in Different Developmental Stages of Lasiopodomys brandtii
Author of the article:LIU Lan1,2, CHEN Yan1,2, LI Ning2, WANG Dawei2, REN Fei2, SONG Ying2, GUO Cong1*, LIU Xiaohui2*
Author's Workplace:1. College of Life Sciences, Sichuan University, Chengdu 610065, China;
2. Key Laboratory of Weed and Rodent Biology and Management, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China
Key Words:Lasiopodomys brandtii; GnRH gene; cloning; gene expression; reproductive regulation
Abstract:Gonadotrophin releasing hormone (GnRH) plays an important role in regulating the reproductive activity of animals. Brandt's vole (Lasperodomys brandtii) is one of the pests in the grassland of eastern Inner Mongolia, China, with obvious seasonal reproduction and its reproductive regulation mechanism is not completely clear. In this study, the cDNA of GnRH gene was cloned from the hypothalamus of L. brandtii, and the mRNA levels of GnRH in different tissues and different ages were measured using qPCR. The results showed that the cloned GnRH cDNA sequence contained an open reading frame of 273 bp, encoding a GnRH precursor of 90 amino acids. Alignment of homologous DNA and amino acid sequences showed that the GnRH of L. brandtii belonged to typeⅠsharing the highest homology with GnRH1 of Microtus ochrogaste. The expression of GnRH gene was detected in hypothalamus, pituitary, testis, adrenal gland, intestine and bladder. The levels of serum testosterone in male voles of 8-week/36-week/80-week were significantly higher than those of 4-week, and the levels of serum estradiol in female voles of 8-week/36-week/80-week were significantly higher than those of 4-week. The highest expression of GnRH was detected in hypothalamus of 80-week voles, and there was no significant difference between 8-week/36-week voles and 4-week voles. We speculated that the GnRH expression of 4-week vole was mainly moderated by the central inhibition, while the GnRH expression of 8-week/36-week vole was mainly moderated by the feedback suppression of steroid. As the age reaching 80 weeks, sex steroid-mediated feedback suppression declined or response time was prolonged, and leading to the high GnRH mRNA level in this stage. These results provided basic data for exploring the reproductive regulation in L. brandtii.
2017,36(5): 498-506 收稿日期:2017-03-11
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