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Issue:ISSN 1000-7083
          CN 51-1193/Q
Director:Sichuan Association for Science and Technology
Sponsored by:Sichuan Society of Zoologists; Chengdu Giant Panda Breeding Research Foundation; Sichuan Association of Wildlife Conservation; Sichuan University
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Your Position :Home->Past Journals Catalog->2017 Vol.36 No.5

Molecular Cloning, Tissue Distribution and Expression of GnRH Gene in Different Developmental Stages of Lasiopodomys brandtii
Author of the article:LIU Lan1,2, CHEN Yan1,2, LI Ning2, WANG Dawei2, REN Fei2, SONG Ying2, GUO Cong1*, LIU Xiaohui2*
Author's Workplace:1. College of Life Sciences, Sichuan University, Chengdu 610065, China;
2. Key Laboratory of Weed and Rodent Biology and Management, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China
Key Words:Lasiopodomys brandtii; GnRH gene; cloning; gene expression; reproductive regulation
Abstract:Gonadotrophin releasing hormone (GnRH) plays an important role in regulating the reproductive activity of animals. Brandt's vole (Lasperodomys brandtii) is one of the pests in the grassland of eastern Inner Mongolia, China, with obvious seasonal reproduction and its reproductive regulation mechanism is not completely clear. In this study, the cDNA of GnRH gene was cloned from the hypothalamus of L. brandtii, and the mRNA levels of GnRH in different tissues and different ages were measured using qPCR. The results showed that the cloned GnRH cDNA sequence contained an open reading frame of 273 bp, encoding a GnRH precursor of 90 amino acids. Alignment of homologous DNA and amino acid sequences showed that the GnRH of L. brandtii belonged to typeⅠsharing the highest homology with GnRH1 of Microtus ochrogaste. The expression of GnRH gene was detected in hypothalamus, pituitary, testis, adrenal gland, intestine and bladder. The levels of serum testosterone in male voles of 8-week/36-week/80-week were significantly higher than those of 4-week, and the levels of serum estradiol in female voles of 8-week/36-week/80-week were significantly higher than those of 4-week. The highest expression of GnRH was detected in hypothalamus of 80-week voles, and there was no significant difference between 8-week/36-week voles and 4-week voles. We speculated that the GnRH expression of 4-week vole was mainly moderated by the central inhibition, while the GnRH expression of 8-week/36-week vole was mainly moderated by the feedback suppression of steroid. As the age reaching 80 weeks, sex steroid-mediated feedback suppression declined or response time was prolonged, and leading to the high GnRH mRNA level in this stage. These results provided basic data for exploring the reproductive regulation in L. brandtii.
2017,36(5): 498-506 收稿日期:2017-03-11
陈雅娟, 张博, 靳铁治, 等. 2014. 不育控制技术在有害鼠类防控中的研究进展[J]. 陕西农业科学, 60(12):73-76.
丁炜东, 曹丽萍, 吴婷婷. 2006. 奥利亚罗非鱼促性腺激素释放激素cDNA的原核表达及其免疫原性研究[J]. 生物工程学报, 22(4):561-566.
Glass JD, 王刚牛. 1988. 雌二醇和孕酮对乏情母羊GnRH免疫反应神经元系统的影响[J]. 草食家畜, (2):26-28.
侯建军, 尹峰. 1996. 关于布氏田鼠的生态学研究[J]. 内蒙古师大学报(自然汉文版), 12(4):54-58.
林浩然. 2004. 促性腺激素释放激素(GnRH)结构与功能及其受体的进化发展[J]. 中山大学学报(自然科学版), 43(6):1-5.
吕江, 张凤琴, 刘汉武, 等. 2013. 具有竞争性繁殖干扰的不育控制害鼠种群模型[J]. 工程数学学报, 30(2):263-270.
母义明. 2004. 下丘脑-垂体轴外GnRH系统研究进展[J]. 武警后勤学院学报(医学版), 13(5):424-425.
任飞, 王大伟, 李宁, 等. 2016. 不同季节出生的布氏田鼠繁殖发育模式分析[J]. 植物保护, 42(2):31-37.
阮玲娟. 2011. 正常动情周期和性腺摘除大鼠外周性激素对下丘脑的影响[D]. 杭州:浙江大学.
施大钊, 海淑珍, 吕东, 等. 1999. 布氏田鼠洞群内社群结构变动与序位的研究[J]. 兽类学报, 19(1):48-55.
宛新荣, 王梦军, 王广和, 等. 2002. 布氏田鼠标志种群的繁殖参数[J]. 兽类学报, 22(2):116-122.
王大伟, 刘祺, 刘明, 等. 2011. EP-1包合物制备及其对布氏田鼠繁殖器官的影响[J]. 兽类学报, 31(1):79-83.
叶丹, 潘建伟, 廖鸣娟. 等. 2003. 促性腺激素释放激素的结构及其生物学功能[J]. 生物化学与生物物理进展, 30(1):49-53.
张洁, 钟文勤. 1979. 布氏田鼠种群繁殖的研究[J]. 动物学报, (3):63-72.
张洁, 钟文勤. 1981. 布氏田鼠洞群内群体结构的研究[J]. 兽类学报, 1(1):53-58.
张洁, 周庆强, 钟文勤. 等. 1978. 布氏田鼠种群年龄的研究[J]. 动物学报, (4):41-55.
张知彬. 1995. 鼠类不育控制的生态学基础[J]. 兽类学报, 15(3):229-234.
Amano M, Takahashi A, Yamanome T, et al. 2002. Molecular cloning of three cDNAs encoding different GnRHs in the brain of barfin flounder[J]. General & Comparative Endocrinology, 126(126):325-333.
Amoss M, Burgus R, Blackwell R, et al. 1971. Purification, amino acid composition and N-terminus of the hypothalamic luteinizing hormone releasing factor (LRF) of ovine origin[J]. Biochemical & Biophysical Research Communications, 44(1):205-210.
Baba Y, Matsuo H, Schally AV. 1971. Structure of the porcine LH-and FSH-releasing hormone. Ⅱ. Confirmation of the proposed structure by conventional sequential analyses[J]. Biochemical & Biophysical Research Communications, 44(2):459-463.
Belchetz PE, Plant TM, Nakai Y, et al. 1979. Hypophysial responses to continuous and intermittent delivery of hypopthalamic gonadotropin-releasing hormone[J]. Science, 202(4368):631-633.
Chen Y, Liu L, Li ZG, et al. 2017. Molecular cloning and characterization of kiss1 in Brandt's voles (Lasiopodomys brandtii)[J]. Biochemistry & Molecular Biology, DOI:10.1016/j.cbp.2017.04.006.
Clarke IJ, Smith JT, Goodman RL, et al. 2009. Kisspeptin and seasonality in sheep[J]. Peptides, 30(1):154-163.
Dubois EA, Zandbergen MA, Peute J, et al. 2002. Evolutionary development of three gonadotropin-releasing hormone (GnRH) systems in vertebrates[J]. Brain Research Bulletin, 57(4):413-418.
Freeman EW, Sammel MD, Lin H, et al. 2006. Associations of hormones and menopausal status with depressed mood in women with no history of depression[J]. Arch Gen Psychiatry, 63(4):375-382.
Gorbman A, Sower SA. 2004. Evolution of the role of GnRH in animal (Metazoan) biology[J]. General & Comparative Endocrinology, 134(3):207-213.
Hood GM, Chesson P, Pech RP. 2000. Biological control using sterilizing viruses:host suppression and competition between viruses in non-spatial models[J]. Journal of Applied Ecology, 37(6):914-925.
Huang W, Yao B, Sun L, et al. 2001. Immunohistochemical and in situ hybridization studies of gonadotropin releasing hormone (GnRH) and its receptor in rat digestive tract[J]. Life Sciences, 68(15):1727-1734.
Lee VHY, Lee LTO, Chow BKC. 2008. Gonadotropin-releasing hormone:regulation of the GnRH gene[J]. Febs Journal, 275(22):5458-5478.
Lethimonier C, Madigou T, Muñoz-Cueto JA. 2004. Evolutionary aspects of GnRHs, GnRH neuronal systems and GnRH receptors in teleost fish[J]. General & Comparative Endocrinology, 135(1):1-16.
Lincoln GA. 1976. Seasonal variation in the episodic secretion of luteinizing hormone and testosterone in the ram[J]. Journal of Endocrinology, 69(2):213-226.
Solomon MB, Herman JP. 2009. Sex differences in psychopathology:of gonads, adrenals and mental illness[J]. Physiology & Behavior, 97(2):250-258.
Swapna I, Sreenivasulu G, Rasheeda MK, et al. 2005. Seabream GnRH:partial cDNA cloning, localization and stage-dependent expression in the ovary of snake head murrel,Channa striatus[J]. Fish Physiology and Biochemistry, 31(2):157-161.
Wang D, Zhang J, Zhang Z. 2012. Effect of testosterone and metatonin on social dominance and agonistic behavior in male Tscheskia triton[J]. Behavioural Processes, 89(3):271-277.
Winter JS, Ellsworth L, Fuller G, et al. 1987. The role of gonadal steroids in feedback regulation of gonadotropin secretion at different stages of primate development[J]. Acta Endocrinologica, 114(2):257-268.
Young KG, Chang JP, Goldberg JI. 1999. Gonadotropin-releasing hormone neuronal system of the freshwater snails Helisoma trivolvis and Lymnaea stagnalis:possible involvement in reproduction[J]. Journal of Comparative Neurology, 404(4):427-437.
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