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Issue:ISSN 1000-7083
          CN 51-1193/Q
Director:Sichuan Association for Science and Technology
Sponsored by:Sichuan Society of Zoologists; Chengdu Giant Panda Breeding Research Foundation; Sichuan Association of Wildlife Conservation; Sichuan University
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Your Position :Home->Past Journals Catalog->2017 Vol.36 No.4

DNA Barcoding in Felidae, and the Effects of Nuclear Mitochondrial Pseudogenes on Species Identification
Author of the article:CAI Yansen1, LI Jialing2, ZHAO Jiao1, ZHANG Liang3*
Author's Workplace:1. Department of Medical Cell Biology and Genetics, Southwest Medical University, Luzhou, Sichuan Province 646000, China;
2. Department of Morphology, Southwest Medical University, Luzhou, Sichuan Province 646000, China;
3. Key Laboratory of Conservation Biology on Endangered Wildlife, Chengdu Research Base of Giant Panda Breeding, Chengdu 610081, China
Key Words:COⅠ; Felidae; nuclear mitochondrial pseudogenes
Abstract:In a variety of animal species, DNA barcoding based on mitochondrial cytochrome c oxidase subunit Ⅰ (COⅠ) gene is an efficient tool for species identification. However, the presence of large mitochondrial pseudogenes in felids may affect the effectiveness of DNA barcoding. In this study, 119 felid samples from 25 species of 12 genera were analyzed.3 pairs of universal primers were used to amplify and sequence the COⅠ genes of 29 felid samples that belonged to 11 species from 6 genera. The results showed that the amplification was failed in 3 samples, nuclear mitochondrial psedogenes (numts) were obtained in 8 samples, and barcode sequences were derived in 18 samples. Combined with the other 93 felid barcode sequences (derived from BOLD Systems), Kimura 2-parameter model was used to calculate the genetic distance and to construct Neighbor-Joining (NJ) tree. The genetic distance within species were 0%-8.1%, with an average of 0.8%; the interspecific genetic distance were 1.4%-13.1%, with an average of 8.7%; and the genetic distance within family were 8.2%-21.8%, with an average of about 15.1%. The NJ tree showed that most of the species formed a monophyletic clade with high confidence (99%), except 3 species; some of the numts sequences could form a clade on NJ tree, and the others were inserted in the branches of the COⅠ sequences, which may compromise the species identification.
2017,36(4): 425-430 收稿日期:2017-03-13
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