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Issue:ISSN 1000-7083
          CN 51-1193/Q
Director:Sichuan Association for Science and Technology
Sponsored by:Sichuan Society of Zoologists; Chengdu Giant Panda Breeding Research Foundation; Sichuan Association of Wildlife Conservation; Sichuan University
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Your Position :Home->Past Journals Catalog->2017 Vol.36 No.4

Regulatory Molecular Mechanism of Periplaneta americana Extracts on Wound Healing Using RNA-seq
Author of the article:CHEN Jiasong1,2,3, CHEN Feng1,2,3, PENG Rui1,2,3, GENG Funeng3*, ZOU Fangdong1,2,3*
Author's Workplace:1. College of Life Sciences, Sichuan University, Chengdu 610064, China;
2. Sichuan Engineering Research Center for Medicinal Animals, Chengdu 610064, China;
3. Sichuan Key Laboratory for Medicinal American Cockroach, Chengdu 610064, China
Key Words:Periplaneta americana; wound healing; RNA-seq; key genes
Abstract:Periplaneta americana is a traditional Chinese medicine, and its extracts have been widely used in the treatment of wound in clinic. However, the molecular mechanism is poorly understood. In this study, to investigate the regulatory mechanism of P. americana extracts on wound healing, a mouse model with excision wound was constructed to assess the efficacy of P. americana extracts on wound therapy. A total of 9 mice were randomly divided into 3 groups:control group (treated with 75% ethanol solution) and 2 P. americana extracts treated groups (Kangfuxin and Jingfen), respectively. RNA-seq was used to screen the key genes in wound skin tissues on day 3. Furthermore, real-time quantitative PCR was performed to confirm the expression of key genes. The results showed that both P. americana extracts could accelerate wound healing. RNA-seq analyses showed that, comparison to the control group, a total of 545 differentially expressed genes (DEGs) were identified in Jingfen treated group and 938 DEGs in Kangfuxin treated group. Bioinformatics analysis revealed that 3 key genes, including Ereg,Gli2 and Tgm3, may play important roles in wound healing during the treatment ofP. americana extracts. Q-PCR experiments further confirmed that Ereg,Gli2 and Tgm3 were up-regulated after the treatment of both P. americana extracts. Collectively, our results indicated that the extracts of P. americana may accelerate wound healing by regulating Ereg, Gli2 and Tgm3 expression.
2017,36(4): 398-403 收稿日期:2017-03-31
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