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Issue:ISSN 1000-7083
          CN 51-1193/Q
Director:Sichuan Association for Science and Technology
Sponsored by:Sichuan Society of Zoologists; Chengdu Giant Panda Breeding Research Foundation; Sichuan Association of Wildlife Conservation; Sichuan University
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Your Position :Home->Past Journals Catalog->2017 Vol.36 No.4

Regulatory Molecular Mechanism of Periplaneta americana Extracts on Wound Healing Using RNA-seq
Author of the article:CHEN Jiasong1,2,3, CHEN Feng1,2,3, PENG Rui1,2,3, GENG Funeng3*, ZOU Fangdong1,2,3*
Author's Workplace:1. College of Life Sciences, Sichuan University, Chengdu 610064, China;
2. Sichuan Engineering Research Center for Medicinal Animals, Chengdu 610064, China;
3. Sichuan Key Laboratory for Medicinal American Cockroach, Chengdu 610064, China
Key Words:Periplaneta americana; wound healing; RNA-seq; key genes
Abstract:Periplaneta americana is a traditional Chinese medicine, and its extracts have been widely used in the treatment of wound in clinic. However, the molecular mechanism is poorly understood. In this study, to investigate the regulatory mechanism of P. americana extracts on wound healing, a mouse model with excision wound was constructed to assess the efficacy of P. americana extracts on wound therapy. A total of 9 mice were randomly divided into 3 groups:control group (treated with 75% ethanol solution) and 2 P. americana extracts treated groups (Kangfuxin and Jingfen), respectively. RNA-seq was used to screen the key genes in wound skin tissues on day 3. Furthermore, real-time quantitative PCR was performed to confirm the expression of key genes. The results showed that both P. americana extracts could accelerate wound healing. RNA-seq analyses showed that, comparison to the control group, a total of 545 differentially expressed genes (DEGs) were identified in Jingfen treated group and 938 DEGs in Kangfuxin treated group. Bioinformatics analysis revealed that 3 key genes, including Ereg,Gli2 and Tgm3, may play important roles in wound healing during the treatment ofP. americana extracts. Q-PCR experiments further confirmed that Ereg,Gli2 and Tgm3 were up-regulated after the treatment of both P. americana extracts. Collectively, our results indicated that the extracts of P. americana may accelerate wound healing by regulating Ereg, Gli2 and Tgm3 expression.
2017,36(4): 398-403 收稿日期:2017-03-31
付小兵, 王德文. 1997. 创伤修复基础[M]. 北京:人民军医出版杜:81.
李远辉, 李希, 冯建安, 等. 2014. 美洲大蠊不同提取部位的体外抑菌活性研究[J]. 中药与临床, 5(6):27-29.
舒崇湘, 程天民, 阎国和, 等. 2001. 6Gy全身照射对皮肤伤口几种细胞外基质成分的影响及W11-a12的促愈作用[J]. 中华创伤杂志, 17(10):604-607.
谭巧云, 满红霞, 那凯歌, 等. 2016. 美洲大蠊提取物对口腔溃疡大鼠模型的作用[J]. 中国临床药理学杂志, 32(11):1014-1016.
伍伟明, 金丽颖, 王真权, 等. 2015. 康复新液治疗肛管难愈合创面的临床研究[J]. 中医药导报, 21(22):71-73.
杨胜群, 熊茜, 邹俊波, 等. 2016. 美洲大蠊水提取物对小鼠烫伤模型的影响[J]. 中国实验方剂学杂志, 22(22):145-149.
曾娟妮, 刘筱, 伍伟明, 等. 2016. 美洲大蠊提取液对大鼠难愈合创面VEGF表达影响的研究[J]. 中国民族民间医药杂志, (19):64-66.
张俊, 孟令贺, 单士军, 等. 2014.康复新液对实验性大鼠皮肤切割伤痂下愈合的影响[J]. 天津医科大学学报, 20(3):192-195.
张晓巍, 朱艳. 2015. 苦参总碱和美洲大蠊提取物对孕激素受体阴性的子宫内膜癌细胞株JEC的作用研究[J]. 中国中药杂志, 40(11):2210-2213.
Ashburner M, Ball CA, Blake JA, et al. 2000. Gene ontology:tool for the unification of biology[J]. Nature Genetics, 25(1):25-29.
Bognar P, Nemeth I, Mayer B, et al. 2014. Reduced inflammatory threshold indicates skin barrier defect in transglutaminase 3 knockout mice[J]. Journal of Investigative Dermatology, 134(1):105-111.
Draper BK, Komurasaki T, Davidson MK, et al. 2003. Epiregulin is more potent than EGF or TGFα in promoting in vitro wound closure due to enhanced ERK/MAPK activation[J]. Journal of Cellular Biochemistry, 89(6):1126-1137.
Kanehisa M, Goto S. 2000. KEGG:kyoto encyclopedia of genes and genomes[J]. Nucleic Acids Research, 28(1):27-30.
Li F, Huang Q, Chen J, et al. 2010. Apoptotic cells activate the "phoenix rising" pathway to promote wound healing and tissue regeneration[J]. Science Signaling, 3(110):ra13.
Luo SL, Huang XJ, Wang Y, et al. 2014. Isocoumarins from American cockroach (Periplaneta americana) and their cytotoxic activities[J].Fitoterapia, 95:115-120.
Martin P, Leibovich SJ. 2005. Inflammatory cells during wound repair:the good, the bad and the ugly[J]. Trends in Cell Biology, 15(11):599-607.
Michalik L, Wahli W. 2006. Involvement of PPAR nuclear receptors in tissue injury and wound repair[J]. The Journal of Clinical Investigation, 116(3):598-606.
Neufert C, Becker C, Türeci Ö, et al. 2013. Tumor fibroblast-derived epiregulin promotes growth of colitis-associated neoplasms through ERK[J]. The Journal of Clinical Investigation, 123(4):1428-1443.
Pan Y, Wang C, Wang B. 2009. Phosphorylation of Gli2 by protein kinase A is required for Gli2 processing and degradation and the Sonic Hedgehog-regulated mouse development[J]. Developmental Biology, 326(1):177-189.
Roy S, Khanna S, Rink C, et al. 2008. Characterization of the acute temporal changes in excisional murine cutaneous wound inflammation by screening of the wound-edge transcriptome[J]. Physiological Genomics, 34(2):162-184.
Tomita K, Haga H, Mizuno K, et al. 2014. Epiregulin promotes the emergence and proliferation of adult liver progenitor cells[J]. American Journal of Physiology-Gastrointestinal and Liver Physiology, 307(1):G50-G57.
Zhang Y, Kobayashi T, Hayashi Y, et al. 2012. Important role of epiregulin in inflammatory responses during corneal epithelial wound healing[J]. Investigative Ophthalmology & Visual Science, 53(4):2414-2423.
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