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Issue:ISSN 1000-7083
          CN 51-1193/Q
Director:Sichuan Association for Science and Technology
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Your Position :Home->Past Journals Catalog->2017 Vol.36 No.4

Population Ecological Genetics of Patelloida pygmaea Based on Mitochondrial 16S rRNA Fragment Sequences in the Bohai Sea
Author of the article:SHI Boyang1, LI Yuanyuan1, WANG Ruxiao1, QU Jiangyong1,2*, GUO Chenghua1*, LIU Chuanlin1, HOU Jianhai1, FANG Hui1
Author's Workplace:1. College of Life Sciences, Yantai University, Yantai, Shandong Province 264005, China;
2. Hainan Key Laboratory for Herpetological Research, Hainan Tropical Ocean University, Sanya, Hainan Province 572000, China
Key Words:Patelloida pygmaea; 16S rRNA; ecological genetical; genetic diversity
Abstract:The relationship between genetic diversity and environmental factors of 15 Patelloida pygmaea populations in the Bohai Sea was studied by PCR and DNA sequencing. A total of 264 polymorphic loci were found in the 16S rRNA partial sequence of the 180 individuals, and 153 transitions as well as 114 transversions were defined by 32 haplotypes. The base contents of C, T, A and G were 18.12%, 27.74%, 33.07%, and 21.07%, respectively. The haplotype diversity was 0.835 1±0.019 1, and the nucleotide diversity was 0.449±0.214. Moreover, the nucleotide diversity of P. pygmaea population in the coast (0.191 8±0.091 7) was higher than that of island (0.107 3±0.081 6), and the results of population demographic history indicated that the populations of P. pygmaea may have experienced a recent demographic expansion. The correlation analysis between environmental factors and genetic factors showed that the genetic distance, sequence variation and nucleotide diversity of P. pygmaea were significantly positive correlated with sunshine duration and sunshine percentage (P<0.05). The result of morphological correlation analysis showed that the main components (length and width) were significantly negative correlated with the annual average precipitation (P<0.05). Average annual precipitation, sunshine duration and sunshine percentage were the dominant factors of P. pygmaea morphological variation, and the population genetic diversity will be higher in the the area with high environmental stablility.
2017,36(4): 386-391 收稿日期:2017-01-12
DOI:10.11984/j.issn.1000-7083.20170013
分类号:Q145;Q346
基金项目:国家自然科学基金地区基金项目(31460562);烟台大学博士科研启动经费(SM15B01);2016年国家级大学生创新创业训练计划项目(201611066009);烟台大学研究生科技创新基金项目;烟台大学开放实验室项目
作者简介:史博洋,硕士研究生,主要从事海洋生物学研究,E-mail:sby_hope@163.com
*通讯作者:曲江勇,E-mail:qjy@ytu.edu.cn;郭承华,E-mail:gch@ytu.edu.cn
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