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Issue:ISSN 1000-7083
          CN 51-1193/Q
Director:Sichuan Association for Science and Technology
Sponsored by:Sichuan Society of Zoologists; Chengdu Giant Panda Breeding Research Foundation; Sichuan Association of Wildlife Conservation; Sichuan University
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Your Position :Home->Past Journals Catalog->2017 Vol.36 No.4

Population Ecological Genetics of Patelloida pygmaea Based on Mitochondrial 16S rRNA Fragment Sequences in the Bohai Sea
Author of the article:SHI Boyang1, LI Yuanyuan1, WANG Ruxiao1, QU Jiangyong1,2*, GUO Chenghua1*, LIU Chuanlin1, HOU Jianhai1, FANG Hui1
Author's Workplace:1. College of Life Sciences, Yantai University, Yantai, Shandong Province 264005, China;
2. Hainan Key Laboratory for Herpetological Research, Hainan Tropical Ocean University, Sanya, Hainan Province 572000, China
Key Words:Patelloida pygmaea; 16S rRNA; ecological genetical; genetic diversity
Abstract:The relationship between genetic diversity and environmental factors of 15 Patelloida pygmaea populations in the Bohai Sea was studied by PCR and DNA sequencing. A total of 264 polymorphic loci were found in the 16S rRNA partial sequence of the 180 individuals, and 153 transitions as well as 114 transversions were defined by 32 haplotypes. The base contents of C, T, A and G were 18.12%, 27.74%, 33.07%, and 21.07%, respectively. The haplotype diversity was 0.835 1±0.019 1, and the nucleotide diversity was 0.449±0.214. Moreover, the nucleotide diversity of P. pygmaea population in the coast (0.191 8±0.091 7) was higher than that of island (0.107 3±0.081 6), and the results of population demographic history indicated that the populations of P. pygmaea may have experienced a recent demographic expansion. The correlation analysis between environmental factors and genetic factors showed that the genetic distance, sequence variation and nucleotide diversity of P. pygmaea were significantly positive correlated with sunshine duration and sunshine percentage (P<0.05). The result of morphological correlation analysis showed that the main components (length and width) were significantly negative correlated with the annual average precipitation (P<0.05). Average annual precipitation, sunshine duration and sunshine percentage were the dominant factors of P. pygmaea morphological variation, and the population genetic diversity will be higher in the the area with high environmental stablility.
2017,36(4): 386-391 收稿日期:2017-01-12
曲江勇, 刘迺发. 2012. 环境因子对雉鸡甘肃亚种种群遗传多样性的影响[J]. 四川动物, 31(4):518-523.
曲若竹, 侯林, 吕红丽, 等. 2004. 群体遗传结构中的基因流[J]. 遗传, 26(3):377-382.
Arbuckle JL. 2010. IBM SPSS Amos 19 user's guide[M]. Chicago:Amos Development Corporation.
Bowen BW, Bass AL, Rocha LA, et al. 2001. Phylogeography of the trumpetfishes(Aulostomus):ring species complex on a global scale[J]. Evolution, 55(5):1029-1039.
Canapa A, Barucca M, Caputo V, et al. 2000. A molecular analysis of the systematics of three Antarctic bivalves[J]. Bolletino di Zoologia, 67(sup1):127-132.
Corre VL, Roux F, Reboud X. 2002. DNA polymorphism at the FRIGIDA gene in Arabidopsis thaliana:extensive nonsynonymous variation is consistent with local selection for flowering time[J]. Molecular Biology & Evolution, 19(8):1261-1271.
Excoffier L, Lischer HEL. 2010. Arlequin suite ver 3.5:a new series of programs to perform population genetics analyses under Linux and Windows[J]. Molecular Ecology Resources, 10(3):564-567.
Finston TL, Peck SB. 1995. Population structure and gene flow in Stomion:a species swarm of flightless beetles of the Galápagos Islands[J]. Heredity An International Journal of Genetics, 75(4):390-397.
Folmer O, Black M, Hoeh W, et al. 1994. DNA primers for amplification of mitochondrial cytochrome c oxidase subunit I from diverse metazoan invertebrates[J]. Molecular Marine Biology & Biotechnology, 3(5):294-299.
Gonzálezwevar CA, Nakano T, Cañete JI, et al. 2010. Molecular phylogeny and historical biogeography of Nacella (Patellogastropoda:Nacellidae) in the Southern Ocean[J]. Molecular Phylogenetics & Evolution, 56(1):115-124.
Grant W, Bowen BW. 1998. Shallow population histories in deep evolutionary lineages of marine fishes:insights from sardines and anchovies and lessons for conservation[J]. Journal of Heredity, 89(5):415-426.
Honda M, Yasukawa Y, Ren H, et al. 2002. Phylogenetic relationships of the Asian box turtles of the genus Cuora sensu lato (Reptilia:Bataguridae) inferred from mitochondrial DNA sequences[J]. Zoologicalence, 19(11):1305-1312.
Huang Z, Liu N, Luo S, et al. 2007. Ecological genetics of rusty-necklaced partridge (Alectoris magna):environmental factors and population genetic variability correlations[J]. Korean Journal of Genetics, 29(2):115-120.
Jin YT, Liu NF. 2008. Ecological genetics of Phrynocephalus vlangalii on the North Tibetan (Qinghai) Plateau:correlation between environmental factors and population genetic variability[J]. Biochemical Genetics, 46(9-10):598-604.
Joos F, Spahni R. 2008. Rates of change in natural and anthropogenic radiative forcing over the past 20,000 years[J]. Proceedings of the National Academy of Sciences of the United States of America, 105(5):1425-1430.
Kim M, Ahn IY, Cheon J, et al. 2009. Molecular cloning and thermal stress-induced expression of a pi-class glutathione S-transferase (GST) in the Antarctic bivalve Laternula elliptica[J]. Comparative Biochemistry & Physiology Part A Molecular & Integrative Physiology, 152(2):207-213.
Kirkendale LA, Meyer CP. 2004. Phylogeography of the Patelloida profunda group (Gastropoda:Lottiidae):diversification in a dispersal-driven marine system[J]. Molecular Ecology, 13(9):2749-2762.
Kumar S, Stecher G, Tamura K. 2016. MEGA7:molecular evolutionary genetics analysis version 7.0 for bigger datasets.[J]. Molecular Biology & Evolution, 33(7):1870.
Librado P, Rozas J. 2009. DnaSP v5[J]. Bioinformatics, 25(11):1451-1452.
Lindberg DR. 1990. Systematics of Potamacmaea fluvia tilis (Blanford):a brackish water patellogastropod (Patelloidinae:Lottiidae)[J]. Journal of Molluscan Studies, 56(2):309-316.
Michod RE. 1981. Ecological genetics[J]. Science, 212(4491):154-155.
Monari M, Matozzo V, Foschi J, et al. 2007. Effects of high temperatures on functional responses of haemocytes in the clam Chamelea gallina[J]. Fish & Shellfish Immunology, 22(1-2):98-114.
Nakai S, Miura O, Maki M, et al. 2006. Morphological and habitat divergence in the intertidal limpet Patelloida pygmaea[J]. Marine Biology, 149(3):515-523.
Nakano T, Ozawa T. 2007. Worldwide phylogeography of limpets of the order Patellogastropoda:molecular, morphological and palaeontological evidence[J]. Journal of Molluscan Studies, 73(1):79-99.
Nevo E. 1981. Genetic variation and climatic selection in the lizard Agama stellio in Israel and Sinai[J]. Theoretical and Applied Genetics, 60(6):369-380.
Ni G, Li Q, Kong L, et al. 2012. Phylogeography of the bivalve Tegillarca granosa in coastal China:implications for management and conservation[J]. Marine Ecology Progress, 452:119-130.
Scheiner SM. 1993. Genetics and evolution of phenotypic plasticity[J]. Annual Review of Ecology & Systematics, 24(1):35-68.
Slatkin M. 1987. Gene flow and the geographic structure of natural populations[J]. Science, 236(4803):787.
Surhone LM, Tennoe MT, Henssonow SF. 2010.Patelloida pygmaea[M]. Saarbrücken:Betascript Publishing.
Takenori S, Takashi O. 1993. Anatomy and systematic position of Yayoiacmea, a new genus for Japanese tiny limpet "Collisella" oyamai Habe, 1955 (Gastropoda:Lottiidae)[J]. The Japanese Journal of Malacology, 52:193-209.
Wayne, Robert K. 2004. Molecular markers, natural history and evolution[M]. Stamford:Sinauer Associates:25-86.
Yu JH, Song JH, Choi MC, et al. 2009. Effects of water temperature change on immune function in surf clams, Mactra veneriformis (Bivalvia:Mactridae)[J]. Journal of Invertebrate Pathology, 102(1):30-35.
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