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Issue:ISSN 1000-7083
          CN 51-1193/Q
Director:Sichuan Association for Science and Technology
Sponsored by:Sichuan Society of Zoologists; Chengdu Giant Panda Breeding Research Foundation; Sichuan Association of Wildlife Conservation; Sichuan University
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Your Position :Home->Past Journals Catalog->2017 Vol.36 No.3

Comparison of mRNA Levels of PPP2CA and PME-1 Genes in the Testes of Yaks and Sterile Cattle-yaks
Author of the article:REN Liang1,2, FU Wei1, JIN Suyu1, HUANG Lin1, LI Caixia1, ZHENG Yucai1*
Author's Workplace:1. College of Life Science and Technology, Southwest University for Nationalities, Chengdu 610041, China;
2. Jiange Animal Disease Control and Prevention Centre, Guangyuan, Sichuan Province 628300, China
Key Words:yak; cattle-yak; hybrid male sterility; protein phosphatase 2A; protein methylesterase 1
Abstract:Protein phosphatase 2A (PP2A) mainly functions in protein reversible phosphorylation, and plays a central role in phosphorylation-dependent signaling pathways. Its activity is regulated by protein methylesterase 1 (PME-1) and other factors. To elucidate the molecular mechanism of hybrid male sterility of yaks, we cloned cDNA sequences of catalytic subunit α (PPP2CA) of PP2A and PME-1 genes. The coding regions of yak PPP2CA and PME-1 genes were 930 bp and 1 143 bp, respectively. The deduced amino acid sequences were conserved. Quantitative PCR assay showed that cattle-yaks (n=7) contained dramatically reduced mRNA levels of PPP2CA and PME-1 genes in testes than yaks (n=13, P<0.01). The present results suggested that the reduced mRNA levels of the two genes in the testes might influence some important signaling pathways and associate with hybrid male sterility of cattle-yaks.
2017,36(3): 306-310 收稿日期:2016-11-30
金帅, 郭宪, 包鹏甲, 等. 2013. 牦牛和犏牛Dmrt7基因序列分析及其在睾丸组织中的表达水平[J]. 中国农业科学, 46(5):1036-1043.
雷杰雯, 黄林, 金素钰, 等. 2016. 牦牛和雄性不育犏牛睾丸ERK1、ERK2、P38基因mRNA水平的比较[J]. 四川动物, 35(5):660-665.
屈旭光, 李齐发, 刘振山, 等. 2008. 牦牛、犏牛睾丸组织中SYCP3基因mRNA表达水平研究[J]. 畜牧兽医学报, 39(8):1132-1136.
Fu W, Li CX, Liu WJ, et al. 2015. Identification of differentially expressed proteins in the testes of normal yaks and sterile hybrids by two-dimensional electrophoresis and mass spectrometry[J]. Indian Journal of Animal Sciences, 85(11):1181-1186.
Hunt T. 2013. On the regulation of protein phosphatase 2A and its role in controlling entry into and exit from mitosis[J]. Advances in Biological Regulation, 53(2):173-178.
Khew-Goodall Y, Mayer RE, Maurer F, et al. 1991. Structure and transcriptional regulation of protein phosphatase 2A catalytic subunit genes[J]. Biochemistry, 30(1):89-97.
Kiely M, Kiely PA. 2015. PP2A:the wolf in sheep's clothing?[J]. Cancers (Basel), 7(2):648-669.
Livak KJ, Schmittgen TD. 2001. Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method[J]. Methods, 25(4):402-408.
Lou YN, Liu WJ, Wang CL, et al. 2014. Histological evaluation and Prdm9 expression level in the testis of sterile male cattle-yaks[J]. Livestock Science, 160(2):208-213.
Ogris E, Du X, Nelson KC, et al. 1999. A protein phosphatase methylesterase (PME-1) is one of several novel proteins stably associating with two inactive mutants of protein phosphatase 2A[J]. Journal of Biological Chemistry, 274(20):14382-14391.
Ortega-Gutiérrez S, Leung D, Ficarro S, et al. 2008. Targeted disruption of the PME-1 gene causes loss of demethylated PP2A and perinatal lethality in mice[J]. PLoS ONE, 3(7):e2486. DOI:10.1371/journal.pone.0002486.
Perrotti D, Neviani P. 2013. Protein phosphatase 2A:a target for anticancer therapy[J]. Lancet Oncology, 14(6):e229-e238.
Rudrabhatla P, Pant HC. 2011. Role of protein phosphatase 2A in Alzheimer's disease[J]. Current Alzheimer Research, 8(6):623-632.
Wlodarchak N, Xing Y. 2016. PP2A as a master regulator of the cell cycle[J]. Critical Reviews in Biochemistry & Molecular Biology, 51(3):162-184.
Xia X, Gholkar A, Senese S, et al. 2015. A LCMT1-PME-1 methylation equilibrium controls mitotic spindle size[J]. Cell Cycle, 14(12):1938-1947.
Yabe R, Miura A, Usui T, et al. 2015. Protein phosphatase methyl-esterase PME-1 protects protein phosphatase 2A from ubiquitin/proteasome degradation[J]. PLoS ONE, 10(12):e0145226. DOI:10.1371/journal.pone.0145226.
Yasutis KM, Kozminski KG. 2013. Cell cycle checkpoint regulators reach a zillion[J]. Cell Cycle, 12(10):1501-1509.
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