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Issue:ISSN 1000-7083
          CN 51-1193/Q
Director:Sichuan Association for Science and Technology
Sponsored by:Sichuan Society of Zoologists; Chengdu Giant Panda Breeding Research Foundation; Sichuan Association of Wildlife Conservation; Sichuan University
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Your Position :Home->Past Journals Catalog->2017 Vol.36 No.3

Effects of Cu2+ Stress on Hemocytes Toxicity and caspase-3, α-2M Gene Expression in Macrobrachium rosenbergii
Author of the article:GUO Hui, ZHU Xiaowen, OU Ronghua, LU Zhicheng, TAN Cuiting, SHEN Yuchun, ZHU Chunhua*
Author's Workplace:Key Laboratory of Marine Ecology and Aquaculture Environment of Zhanjiang, College of Fisheries, Guangdong Ocean University, Zhanjiang, Guangdong Province 524025, China
Key Words:Macrobrachium rosenbergii; Cu2+; hemocytes toxicity; gene expression; apoptosis
Abstract:The present study analyzed the toxic effects of Cu2+ on apoptotic cell ratio, ROS production, esterase activities, NO production, and the expression levels of caspase-3 and α-2M in hemocytes of Macrobrachium rosenbergii. The results showed that the changes of apoptotic cell ratio were consistent with ROS production which was increased significantly (P<0.05) after exposure for 6~48 h. Esterase activities were significantly decreased (P<0.05) at 3~48 h and the minimum was observed at 48 h. The NO production continued increasing and the level became significant difference (P<0.05) after exposure for 6~48 h. The relative expression levels of caspase-3 were significantly induced (P<0.05) after exposure for 6~48 h, and reached the peak at 24 h. The relative expression of α-2M was significantly increased (P<0.05) and reached the peak at 12 h. Moreover, the expression level of α-2M reduced after exposure for 24 h and became significantly different(P<0.05) at 48 h. Correlation analysis showed that significant positive correlation (P<0.001) between apoptotic cell ratio and ROS production was observed, as well as apoptotic cell ratio and NO production. All these results suggested that esterase activities were significantly inhibited after Cu2+ exposure, while NO and ROS production as well as α-2M expression were induced against Cu2+ exposure. Along with stress time increasing, overfull production of ROS and NO caused oxidative stress to prawn and then induced the expression level of caspase-3 which finally resulted in apoptosis, indicating that Cu2+ stress may have immunosuppressive and toxic effects to M. rosenbergii, and the increased production of NO and ROS might be the main cause of apoptosis.
2017,36(3): 293-299 收稿日期:2017-02-08
郭慧, 冼健安, 王安利. 2015. 亚硝酸盐对凡纳滨对虾血细胞毒性及p53基因表达的影响[J]. 水生态学杂志, 36(2):61-67.
国家海洋局. 1989. 渔业水质标准[M]. 北京:中国标准出版社.
汪义军. 1997. 体内一氧化氮含量测定的几种方法[J]. 国际检验医学杂志, (1):7-9.
吴维福, 陈娈娈, 李郁娇, 等. 2014. 三丁基锡对罗氏沼虾血清中免疫酶活力的影响[J]. 广东海洋大学学报, 34(3):17-21.
冼健安, 钱坤, 郭慧, 等. 2015. 杂色鲍血细胞分类、结构和免疫功能的流式细胞术分析[J]. 海洋科学, 39(12):8-14.
Begum R, Bhadra SC, Shahjahan R, et al. 2008. Esterase banding pattern in different tissues of Pangasius hypophthalmus (Sauvage, 1878)[J]. Bangladesh Journal of Zoology, 36:287-294.
Bharadwaj AS, Patnaik S, Browdy CL, et al. 2014. Comparative evaluation of an inorganic and a commercial chelated copper source in Pacific white shrimp Litopenaeus vannamei (Boone) fed diets containing phytic acid[J]. Aquaculture, 422-423(3):63-68.
Brown GC. 2010. Nitric oxide and neuronal death[J]. Nitric Oxide, 23(3):153-165.
Chang CC, Yeh MS, Cheng W. 2009. Cold shock-induced norepinephrine triggers apoptosis of haemocytes via caspase-3 in the whiteshrimp, Litopenaeus vannamei[J]. Fish & Shellfish Immunology, 27(6):695-700.
Chen W, Wang CH. 2001. The susceptibility of the giant freshwater prawn Macrobrachium rosenbergii to Lactococcus garvieae and its resistance under copper sulfate stress[J]. Diseases of Aquatic Organisms, 47(2):137-144.
Frías-Espericueta MG, Castro-Longoria R, Barrón-Gallardo GJ, et al. 2008. Histological changes and survival of Litopenaeus vannamei juveniles with different copper concentrations[J]. Aquaculture, 278(1-4):97-100.
Gill SS, Tuteja N. 2010. Reactive oxygen species and antioxidant machinery in abiotic stress tolerance in crop plants[J]. Plant Physiology and Biochemistry, 48(12):909-930.
Gonias SL. 1992. Alpha 2-macroglobulin:a protein at the interface of fibrinolysis and cellular growth regulation[J]. Experimental Hematology, 20(3):302-311.
Guo H, Miao YT, Xian JA, et al. 2015. Expression profile of antioxidant enzymes in hemocytes from freshwater prawn Macrobrachium rosenbergii exposed to an elevated level of copper[J]. Bulletin of Environmental Contamination and Toxicology, 95(4):447-451.
Guo H, Xian JA, Li B, et al. 2013. Gene expression of apoptosis-related genes, stress protein and antioxidant enzymes in hemocytes of white shrimp Litopenaeus vannamei under nitrite stress[J]. Comparative Biochemistry and Physiology Part C:Toxicology & Pharmacology, 157(4):366-371.
Havanapan PO, Taengchaiyaphum S, Ketterman AJ, et al. 2016. Yellow head virus infection in black tiger shrimp reveals specific interaction with granule-containing hemocytes and crustinPm1 as a responsive protein[J]. Developmental & Comparative Immunology, 54(1):126-136.
Ho PY, Cheng CH, Cheng W. 2009. Identification and cloning of the α2-macroglobulin of giant freshwater prawn Macrobrachium rosenbergii and its expression in relation with the molt stage and bacteria injection[J]. Fish & Shellfish Immunology, 26(3):459-466.
Liao CM, Chang CF, Yeh CH, et al. 2006. Metal stresses affect the population dynamics of disease transmission in aquaculture species[J]. Aquaculture, 257(1-4):321-332.
Lorenzon S, de Guarrini S, Smith VJ, et al. 1999. Effects of LPS injection on circulating haemocytes in crustaceans in vivo[J]. Fish & Shellfish Immunology, 9(1):31-50.
Mahmood K, Yang JS, Chen D, et al. 2009. Response of metallothionein gene-1 to laboratory exposure to heavy metals and thermal stress in the freshwater prawn Macrobrachium rosenbergii[J]. Journal of Hazardous Materials, 167(1-3):523-530.
Martínez A, Romero Y, Castillo T, et al. 2014. The effect of copper on the color of shrimps:redder is not always healthier[J]. PLoS ONE, 9(9):e107673.DOI:10.1371/journal.pone.0107673.
Perazzolo LM, Bachère E, Rosa RD, et al. 2011. Alpha2-macroglobulin from an Atlantic shrimp:biochemical characterization, sub-cellular localization and gene expression upon fungal challenge[J]. Fish & Shellfish Immunology, 31(6):938-943.
Rao MS, Anjaneyulu N. 2008. Effect of copper sulfate on molt and reproduction in shrimp Litopenaeus vannamei[J]. International Journal of Biological Chemistry, 2(1):35-41.
Rashid MA, Begum RA, Shahzahan RM. 2013. Tissue distribution of esterase isozymes and their responses to cypermethrin in three macrobrachium species[J]. Journal of the Asiatic Society of Bangladesh, Science, 38(2):227-235.
Rijiravanich A, Browdy CL, Withyachumnarnkul B. 2008. Knocking down caspase-3 by RNAi reduces mortality in Pacific white shrimp penaeus Litopenaeus vannamei challenged with a low dose of white-spot syndrome virus[J]. Fish & Shellfish Immunology, 24(3):308-313.
Santos MHS, Cunha NTD, Bianchini A. 2000. Effects of copper and zinc on growth, feeding and oxygen consumption of Farfantepenaeus paulensis postlarvae (Decapoda:Penaeidae)[J]. Journal of Experimental Marine Biology and Ecology, 247(2):233-242.
Scheler C, Durner J, Astier J. 2013. Nitric oxide and reactive oxygen species in plant biotic interactions[J]. Current Opinion in Plant Biology, 16(4):534-539.
Shanthi S, Vaseeharan B. 2014. Alpha 2 macroglobulin gene and their expression in response to GFP tagged Vibrio parahaemolyticus and WSSV pathogens in Indian white shrimp Fenneropenaeus indicus[J]. Aquaculture, 418-419(1):48-54.
Silva-Aciares F, Moraga D, Riquelme C. 2013. Effect of copper on the immunomodulatory activities of haemocytes in juveniles of the abalone Haliotis rufescens cultivated under hatchery conditions[J]. Aquaculture, 410-411:72-78.
Simon HU, Haj-Yehia A, Levi-Schaffer F. 2000. Role of reactive oxygen species (ROS) in apoptosis induction[J]. Apoptosis, 5(5):415-418.
Valavanidis A, Vlachogianni T. 2012. Metal pollution in ecosystems. Ecotoxicology studies and risk assessment in the marine environment[J]. Science Advances on Environment, Toxi-Cology & Ecotoxicology Issues.
Wang SL, Xu XR, Sun YX, et al. 2013. Heavy metal pollution in coastal areas of south China:a review[J]. Marine Pollution Bulletin, 76(1-2):7-15.
Wang ZH, Feng J, Nie XP. 2015. Recent environmental changes reflected by metals and biogenic elements in sediments from the Guishan Island, the Pearl River Estuary, China[J]. Estuarine Coastal & Shelf Science, 164(1-April):493-505.
Xian JA, Li B, Guo H, et al. 2014. Haemocyte apoptosis of the tiger shrimp Penaeus monodon exposed to cadmium[J]. Bulletin of Environmental Contamination and Toxicology, 92(5):525-528.
Xian JA, Miao YT, Li B, et al. 2013. Apoptosis of tiger shrimp (Penaeus monodon) haemocytes induced by Escherichia coli lipopolysaccharide[J]. Comparative Biochemistry and Physiology Part A:Molecular & Integrative Physiology, 164(2):301-306.
Xian JA, Wang AL, Ye CX, et al. 2010. Phagocytic activity, respiratory burst, cytoplasmic free-Ca2+ concentration and apoptotic cell ratio of haemocytes from the black tiger shrimp, Penaeus monodon under acute copper stress[J]. Comparative Biochemistry and Physiology Part C:Toxicology & Pharmacology, 152(2):182-188.
Yang Y, Bazhin AV, Werner J, et al. 2013. Reactive oxygen species in the immune system[J]. International Reviews of Immunology, 32(3):249-270.
Yeh ST, Liu CH, Chen JC. 2004. Effect of copper sulfate on the immune response and susceptibility to Vibrio alginolyticus in the white shrimp Litopenaeus vannamei[J]. Fish & Shellfish Immunology, 17(5):437-446.
Zvereva E, Serebrov V, Glupov V, et al. 2003. Activity and heavy metal resistance of non-specific esterases in leaf beetle Chrysomela lapponica from polluted and unpolluted habitats[J]. Comparative Biochemistry and Physiology Part C:Toxicology & Pharmacology, 135(4):383-391.
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