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Issue:ISSN 1000-7083
          CN 51-1193/Q
Director:Sichuan Association for Science and Technology
Sponsored by:Sichuan Society of Zoologists; Chengdu Giant Panda Breeding Research Foundation; Sichuan Association of Wildlife Conservation; Sichuan University
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Your Position :Home->Past Journals Catalog->2017 Vol.36 No.2

Improved Experimental Techniques of Blood Collection from the Submandibular Vein Plexus in Mice
Author of the article:TAI Xuejiao1, GUO Xingrong2, LUO Chao1, SHAN Mengye1, LIU Xin1, RUAN Xuzhi1*
Author's Workplace:1. Faculty of Basic Medical Sciences, Hubei University of Medicine, Shiyan, Hubei Province 442000, China;
2. Hubei Key Laboratory of Embryonic Stem Cell Research, Taihe Hospital, the Affiliated Hospitals of Hubei University of Medicine, Shiyan, Hubei Province 442000, China
Key Words:mouse; blood collection; submandibular vein; blood collection from the submandibular vein plexus
Abstract:Objective To introduce a simple and repeatable blood collection method from the submandibular vein plexus of mice. The operators can collect a large amount of blood by using this method. Methods We first chose the best point of blood collection by anatomical observation of facial blood vessels in mice, then the microcapillary pipets were used to collect blood quickly and efficiently. About 20 SPF ICR male mice with a weight range of 18-22 g were selected, and the blood was collected from the submandibular point using the microcapillary pipet. Results Appropriately, a volume of 0.5-0.7 mL blood could be collected from each mouse within 40-60 s by one operator. Conclusion The improved blood collection method had the advantages of smaller trauma to the experimental mice and can collect more blood. In addition, the operation process can be easily repeated. This method was particularly suitable for the beginners or people who needed large amounts of blood from mice. This improved blood collect method was an easy, safe, fast and efficient method to get more blood from mice, which should become an extensively used method.
2017,36(2): 211-214 收稿日期:2016-07-28
DOI:10.11984/j.issn.1000-7083.20160201
分类号:R-33
基金项目:湖北省自然科学基金项目(2015CFC812);湖北十堰市社发指导项目(16y03)
作者简介:台雪姣(1990-),女,硕士研究生,研究方向:肿瘤病理学,E-mail:taixuejiao0109@163.com
*通讯作者:阮绪芝,博士,教授,硕士生导师,研究方向:肿瘤分子生物学,E-mail:ruanxuzhi117@163.com
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