Latest Cover

Online Office

Contact Us

Issue:ISSN 1000-7083
          CN 51-1193/Q
Director:Sichuan Association for Science and Technology
Sponsored by:Sichuan Society of Zoologists; Chengdu Giant Panda Breeding Research Foundation; Sichuan Association of Wildlife Conservation; Sichuan University
Address:College of Life Sciences, Sichuan University, No.29, Wangjiang Road, Chengdu, Sichuan Province, 610064, China
Fax:+86-28-85410485 &
Your Position :Home->Past Journals Catalog->2017 Vol.36 No.2

Influence of Periplaneta americana Extract on Antioxidant Stress in Rats
Author of the article:SUI Shiyan, GE Yanan, XU Quwei, SU Jingjing
Author's Workplace:College of Agronomy and Bioscience, Dali University, Dali, Yunnan Province 671003, China
Key Words:Periplaneta americana alcohol extract; oxidative stress; gene expression; serum; ovary; rats
Abstract:Objective To study the effect of Periplaneta americana alcohol extract on antioxidant index of serum and ovary in rats. Methods A total of 17 adult female SD rats were randomly assigned to 3 groups after a week of adaption in the laboratorial environment. The rats of control group (n=5) were fed with physiological saline (9 mL·kg-1) through stomach perfusion. The rats of experimental groups (6 per group) were treated with low-dose (9 mL·kg-1, 0.03 g·mL-1) and high-dose (9 mL·kg-1, 0.09 g·mL-1) P. americana alcohol extract as described above. Rats were treated every other day for 30 days. Subsequently, the serum and ovaries of rats were collected, and the levels of malondialdehyde (MDA), superoxide dismutase (SOD), catalase (CAT), nitric oxide (NO), glutathione (GSH-PX) in the serum, the expression of gene SOD, GSH-PX and CAT in the ovary were detected. Results Compared with control group, the NO content in rats serums of high-dose group (P=0.085) and low-dose group (P=0.091) showed a decreasing trend but not significant; the content of SOD in rats serums were significantly increased (P<0.05). Moreover, the content of MDA in rats serums were significantly decreased in the high-dose group (P<0.05). In addition, P. americana alcohol extract treatment significantly increased the expression of SOD gene in ovary (P<0.05). Conclusion P. americana alcohol extract can significantly increase the content of antioxidant index SOD and reduce levels of the oxidative stress indicators NO and MDA, and can also significantly increase the expression of SOD gene in ovary. Therefore, P. americana alcohol extract has a certain antioxidant stress function to the ovary of rats.
2017,36(2): 198-202 收稿日期:2016-10-14
戴云, 曾茗, 项朋志. 2005. 蜚蠊的药用价值[J]. 中药材, 28(9):848-850.
甘平, 张旭强, 何旭, 等. 2011. 美洲大蠊醇提物对小鼠急性肝损伤的保护作用[J]. 现代药物与临床, 26(2):123-128.
何正春, 胡明辉, 王晓雨, 等. 2009. 美洲大蠊提取物对3株人及小鼠白血病细胞的细胞毒性研究[J]. 云南中医中药杂志, 30(5):56-57.
焦春香, 张成桂, 刘光明, 等. 2011. 美洲大蠊提取物中抗衰老活性部位抗氧化活性的初步分析[J]. 时珍国医国药, 22(6):1389-1391.
吴军, 赵凤鸣, 王明艳, 等. 2007. 四君子汤对环磷酰胺造模小鼠血清中SOD、GSH、CAT和MDA水平的影响[J]. 辽宁中医杂志, 34(8):1168-1169.
邹俊波, 桑文涛, 王芳, 等. 2016. 美洲大蠊提取物对乙醇致小鼠急性胃溃疡的预防作用[J]. 中成药, 38(11):2325-2331.
Agarwal A, Gupta S, Sharma RK. 2005. Role of oxidative stress in female reproduction[J]. Reproductive Biology and Endocrinology, 3(28):1-21.
Agarwal A, Gupta S, Sikka S. 2006. The role of free radicals and antioxidants in reproduction[J]. Current Opinion in Obstetrics and Gynecology, 18(3):325-332.
Agarwal A, Saleh RA, Bedaiwy MA. 2003. Role of reactive oxygen species in the pathophysiology of human reproduction[J]. Fertility and Sterility, 79(4):829-843.
Aitken RJ,Koppers AJ. 2011. Apoptosis and DNA damage in human spermatozoa[J]. Asian Journal of Andrology, 13(1):36-42.
Bagchi D, Garg A, Krohn RL, et al. 1997. Oxygen free radical scavenging abilities of vitamins C and E and a grape seed proanthocyanidin extract in vitro[J]. Research Communications in Molecular Pathology and Pharmacology, 95(2):179-189.
Bausenwein J, Serke H, Eberle K, et al. 2010. Elevated levels of oxidized low-density lipoprotein and of catalase activity in follicular fluid of obese women[J]. Molecular Human Reproduction, 16(2):117-124.
Goud AP, Goud PT, Diamond MP, et al. 2008. Reactive oxygen species and oocyte aging:role of superoxide, hydrogen peroxide, and hypochlorous acid[J]. Free Radical Biology and Medicine, 44(7):1295-1304.
Husain K, Whitworth C, Somani SM, et al. 2001. Carboplatin-induced oxidative stress in rat cochlea[J]. Hearing Research, 159(1-2):14-22.
Livak KJ, Schmittgen TD. 2001. Analysis of relative gene expression data using real-time quantitative PCR and the 2-ΔΔCT method[J]. Methods, 25(4):402-408.
Oyawoye O, Abdel Gadir A, Garner A, et al. 2003. Antioxidants and reactive oxygen species in follicular fluid of women undergoing IVF:relationship to outcome[J]. Human Reproduction, 18(11):2270-2274.
Riva C, Binelli A, Cogni D, et al. 2007. Evaluation of DNA damage induced by decabromodiphenyl ether (BDE-209) in hemocytes of dreissena polymorpha using the comet and micronucleus assays[J]. Environmental and Molecular Mutagenesis, 48(9):735-743.
Rizzo A, Roscino MT, Binetti F, et al. 2012. Roles of reactive oxygen species in female reproduction[J]. Reproduction in Domestic Animals, 47(2):344-352.
Sikka SC. 2001. Relative impact of oxidative stress on male reproductive function[J]. Current Medicinal Chemistry, 8(7):851-862.
Wathes DC, Abayasekara DR, Aitken RJ. 2007. Polyunsaturated fatty acids in male and female reproduction[J]. Biology of Reproduction, 77(2):190-201.
CopyRight©2022 Editorial Office of Sichuan Journal of Zoology 蜀ICP备08107403号-3