This study aims to investigate the potential protective effect of Ploygonum multiflorum
Thunb. glycoside (PM) on the ability of learning and memory and the regulatory function on the expression of ChAT and AChE in the hippocampus of subacute aging mice model.Methods
Kunming (KM) mice were treated by AlCl3
) via intragastric administration (Ig) and D-glactose (120 mg·kg-1
) via subcutaneous injection on neck for 60 days. The qualified individuals were screened by jumping-out experiment. Male and female qualified mice were equally divided into6 groups, including normal control group, disease model group (negative control), estradiol positive group (10 mg·kg-1
), PM-high dose (80 mg·kg-1
), PM-medium dose (40 mg·kg-1
) and PM-low dose (20 mg·kg-1
) groups. Negative control was treated by saline buffer; healthy mice treated by saline buffer were used as normal control. KM mice were treated by PM or estradiol via subcutaneous injection on neck for 15 days since the 15th day after the model was established. The learning and memory ability of mice in each group was determined by the jumping-out experiment. ChAT and AChE protein expression in the hippocampus tissue were detected by Western Blot assay.Results
Compared with normal control group, the learning and memory ability of mice decreased in the treatment group, and the number of avoidance errors was significantly increased (P
<0.05). Compared with the disease model group, the learning and memory ability of mice treated by designated dose of PM and estradiol was significantly improved, and the number of withdrawal avoidance errors was obviously or extreme obviously decreased (P
<0.05 or P
<0.001). However, the data of estradiol positive group was similar with those of PM treatment groups (P
>0.05). Compared with the disease model group, the expression of ChAT and AChE was down-regulated in the hippocampus of groups treated by PM in different dose or estradiol (P
<0.05 or P
PM can effectively improve the learning and memory ability of the subacute aging model mice. The mechanism is related with the increased level of ACh in the hippocampus which can protect the cholinergic system.
2017,36(1): 48-53 收稿日期：2016-10-09
马允, 张树球, 梁月秀, 等. 2005. 乌圆补血口服液对拟老年痴呆症小鼠脑乙酰胆碱酯酶的影响[J]. 中国临床康复, 44:94-96.
梅雪, 余刘勤, 陈小云, 等. 2016. 何首乌化学成分和药理作用的研究进展[J]. 药物评价研究, 39(1):122-131.
仇淑君, 王忠良, 李广意. 2014. 丹参对AD模型小鼠学习记忆及脑内和血清内乙酰胆碱酯酶含量的影响[J]. 实验动物科学, 31(4):11-15.
孙荣花, 李爽, 邵莹, 等. 2015. D-半乳糖致小鼠亚急性衰老模型方法优化[J]. 中药药理与临床, 31(4):293-295.
王昱, 王胜青, 何九军, 等. 2015. 橄榄苦苷对阿尔茨海默病小鼠模型学习记忆能力的影响[J]. 中国新药杂志, 24(14):1654-1658.
张兰, 李林, 李雅莉. 2004. 何首乌有效成分二苯乙烯甙对神经细胞保护作用的机制[J]. 中国临床康复, 8(2):118-120.
张玉莲, 周震, 韩文文, 等. 2014. 何首乌有效成分二苯乙烯苷对Aβ25-31诱导神经干细胞定向分化的影响[J]. 中医杂志, 55(4):323-327.
Auld DS, Kornecook TJ, Bastianetto S, et al. 2002. Alzheimer's disease and the basal forebrain cholinergic system:relations to beta-amyloid peptides, cognition, and treatment strategies[J]. Progress in Neurobiology, 68(3):209-245.
Ballard C, Gauthier S, Corbett A, et al.1995. Alzheimer's disease[J]. Lancet, 377:1019-1031.
Ikonomovic MD, Abrahamson EE, Isanski BA, et al. 2007. Superior frontal cortex cholinergic axon density in mild cognitive impairment and early Alzheimer disease[J]. Archives of Neurology, 64(9):1312-1317.
Karran E, Mercken M, De Strooper B. 2011. The amyloid cascade hypothesis for Alzheimer's disease:an appraisal for the development of herapeutics[J]. Nature Reviews Drug Discovery, 10(9):698-712.
Li Q, Chen M, Liu H, et al. 2012. Expression of APP, BACE1, AChE and ChAT in an AD model in rats and the effect of donepezil hydrochloride treatment[J]. Molecular Medicine Report, 6(6):1450-1454.
Orgogozo JM, Rigaud AS, Stöffler A, et al. 2002. Efficacy and safety of memantine in patients with mild to moderate vascular dementia:a randomized, placebo-controlled trial (MMM 300)[J]. Stroke, 33(7):1834-1839.
Xiao F, Li XG, Zhang XY, et al. 2011. Combined administration of D-galactose and aluminium induces Alzheimer-like lesions in brain[J]. Neuroscience Bulletin, 27(3):143-155.
Yang WN, Han H, Hu XD, et al. 2013. The effects of perindopril on cognitive impairment induced by d-galactose and aluminum trichloride via inhibition of acetylcholinesterase activity and oxidative stress[J]. Pharmacology Biochemistry and Behavior, 114:31-36.
Yang XP, Liu TY, Qin XY, et al. 2014. Potential protection of 2, 3, 5, 4'-tetrahydroxystilbene-2-O-β-D-glucoside against staurosporine-induced toxicity on cultured rat hippocampus neurons[J]. Neuroscience Letters, 576(2):79-83.