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Issue:ISSN 1000-7083
          CN 51-1193/Q
Director:Sichuan Association for Science and Technology
Sponsored by:Sichuan Society of Zoologists; Chengdu Giant Panda Breeding Research Foundation; Sichuan Association of Wildlife Conservation; Sichuan University
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Your Position :Home->Past Journals Catalog->2017 Vol.36 No.1

The Effect of Ploygonum multiflorum Thunb. Glycoside on Learning and Memory and Expression of ChAT and AChE in the Hippocampus of Subacute Aging Model Mice
Author of the article:YU Yun1,2#, WANG Baili1,3#, ZHANG Yu1,2#, ZHAO Yu1,2*, QIN Xiaoyan1,2*
Author's Workplace:1. College of Life & Environmental Science, Minzu University of China, Beijing 100081, China;
2. Beijing Engineering Research Center of Food Environment and Health, Minzu University of China, Beijing 100081, China;
3. The School Hospital Clinical Laboratory, Minzu University of China, Beijing 100081, China
Key Words:Polygonum multiflorum Thunb. glycoside; subacute aging model mice; learning and memory; ChAT; AChE
Abstract:Objective This study aims to investigate the potential protective effect of Ploygonum multiflorum Thunb. glycoside (PM) on the ability of learning and memory and the regulatory function on the expression of ChAT and AChE in the hippocampus of subacute aging mice model.Methods Kunming (KM) mice were treated by AlCl3 (10 mg·kg-1) via intragastric administration (Ig) and D-glactose (120 mg·kg-1) via subcutaneous injection on neck for 60 days. The qualified individuals were screened by jumping-out experiment. Male and female qualified mice were equally divided into6 groups, including normal control group, disease model group (negative control), estradiol positive group (10 mg·kg-1), PM-high dose (80 mg·kg-1), PM-medium dose (40 mg·kg-1) and PM-low dose (20 mg·kg-1) groups. Negative control was treated by saline buffer; healthy mice treated by saline buffer were used as normal control. KM mice were treated by PM or estradiol via subcutaneous injection on neck for 15 days since the 15th day after the model was established. The learning and memory ability of mice in each group was determined by the jumping-out experiment. ChAT and AChE protein expression in the hippocampus tissue were detected by Western Blot assay.Results Compared with normal control group, the learning and memory ability of mice decreased in the treatment group, and the number of avoidance errors was significantly increased (P<0.05). Compared with the disease model group, the learning and memory ability of mice treated by designated dose of PM and estradiol was significantly improved, and the number of withdrawal avoidance errors was obviously or extreme obviously decreased (P<0.05 or P<0.001). However, the data of estradiol positive group was similar with those of PM treatment groups (P>0.05). Compared with the disease model group, the expression of ChAT and AChE was down-regulated in the hippocampus of groups treated by PM in different dose or estradiol (P<0.05 or P<0.001).Conclusion PM can effectively improve the learning and memory ability of the subacute aging model mice. The mechanism is related with the increased level of ACh in the hippocampus which can protect the cholinergic system.
2017,36(1): 48-53 收稿日期:2016-10-09
DOI:10.11984/j.issn.1000-7083.20160272
分类号:Q95-33
基金项目:国家自然科学基金项目(31372225);中央高校自主科研基金项目(2016SHXY01);社会医学学术团队建设项目(2015MDTD13C);北京市大学生创新项目(URTP2015110019)
作者简介:俞云(1993-),E-mail:yuyun2012446075@139.com;汪佰莉(1979-),E-mail:13811636247@163.com;张雨(1993-),E-mail:2112015746@qq.com
*通讯作者:赵宇,E-mail:zhaoyu198387@gmail.com;覃筱燕,E-mail:bjqinxiaoyan@muc.edu.cn
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