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Issue:ISSN 1000-7083
          CN 51-1193/Q
Director:Sichuan Association for Science and Technology
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Your Position :Home->Past Journals Catalog->2016 Vol.35 No.6

Effects of Sulfide Stress on Expressions of Antioxidant Enzymes in Litopenaeus vannamei Haemocytes
Author of the article:WANG Lei1, ZHANG Xiuxia2, ZHENG Peihua1, LU Yaopeng1, XIAN Jian'an2*
Author's Workplace:1. Institute of Pharmaceutical Research, South China Normal University, Guangzhou 510631, China;
2. Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, China
Key Words:sulfide; Litopenaeus vannamei; haemocyte; antioxidant enzyme; gene expression
Abstract:Sulfide is one of the common water pollutants in aquaculture. To investigate the role of antioxidant enzymes in anti-sulfide defense of shrimp haemocytes, gene expression levels of copper, zinc-superoxide dismutase (Cu, Zn-SOD), manganese-superoxide dismutase (Mn-SOD), catalase (CAT), glutathion peroxidase (GPx) and thioredoxin (TRx) were determined in haemocytes of the white shrimp (Litopenaeus vannamei) exposure to sulfide (0 mg·L-1 and 2.0 mg·L-1) for 0 h, 6 h, 12 h, 24 h and 48 h. The results showed that expression levels of Cu, Zn-SOD and TRx significantly increased after 6~24 h exposure, and reduced to the control levels at 48 h. Mn-SOD and GPx mRNA expression levels were up-regulated after 6~48 h exposure. CAT expression level began to increase after 12 h exposure. These results indicated that the mRNA expressions of antioxidant enzymes were induced to protect the haemocytes against sulfide stress. Cu, Zn-SOD, Mn-SOD, GPx and TRx displayed high sensitivity to sulfide stress, and might play the dominant role at the initial stage of sulfide stress, whereas CAT mainly worked at the later period.
2016,35(6): 884-888 收稿日期:2016-08-25
DOI:10.11984/j.issn.1000-7083.20160229
分类号:X503.225;Q959.223
基金项目:国家自然科学基金项目(31500326);广东省自然科学基金项目(2014A030310185);广东省普通高校青年创新人才项目(2014KQNCX056);广东省普通高校特色创新项目(2015KTSCX019);华南师范大学青年教师科研培育基金项目(13KJ16)
作者简介:汪蕾(1986-),女,讲师,主要从事水产养殖生态及毒理学研究,E-mail:wanglei@scnu.edu.cn
*通讯作者:冼健安,E-mail:xian-ja@163.com
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