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Issue:ISSN 1000-7083
          CN 51-1193/Q
Director:Sichuan Association for Science and Technology
Sponsored by:Sichuan Society of Zoologists; Chengdu Giant Panda Breeding Research Foundation; Sichuan Association of Wildlife Conservation; Sichuan University
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Your Position :Home->Past Journals Catalog->2016 Vol.35 No.6

Genetic Diversity of Lasiopodomys brandtii Populations in Inner Mongolia
Author of the article:WEI Lei1,3, ZHAO Suhua2, HU Yi3, WANG Chengmin3, HE Hongxuan3*
Author's Workplace:1. Bozhou University, Bozhou, Anhui Province 236800, China;
2. Suzhou Vocational Technology College, Suzhou, Anhui Province 234101, China;
3. National Research Center for Wildlife Born Diseases, Key Laboratory of Animal Ecology and Conservation Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China
Key Words:Lasiopodomys brandtii; MHC class Ⅱ gene; genetic diversity
Abstract:The genetic diversity of 8 geo-populations of Lasiopodomys brandtii were analyzed using PCR-RFLP and sequence analysis based on MHC class Ⅱ exon 2. The results showed that 6 alleles, 21 haplotypes and 13 restriction enzyme polymorphic loci were detected, and 3 haplotypes were shared by all the populations. The Chi-square test results suggested that the 8 populations of L. brandtii in the present study did not follow with the Hardy-Weinberg equilibrium at six restriction enzyme polymorphic loci. Sequence analysis showed that there were 57 bases mutations. Haplotype diversity (H) and nucleotide diversity (πn) were 0.746 5~0.873 3 and 0.006 06~0.016 55, respectively. Phylogenetic analyses suggested that the sampled individuals were clustered into three clades:populations of Xilinhaote, Erlianhaote, Dongwuqi, and Xiwuqi were clustered as Zone A; populations of Xinbaerhuzuoqi, Chenbaerhuqi and Xinbaerhuyouqi were clustered as Zone B; while the Zone C was comprised of the sole population from Zhengxiangbaiqi. And this clustering pattern was consistent with their geographical distributions. The result of AMOVA analysis showed that regional genetic variation was accounted for 64.08% of the total, intraregional population variation was accounted for 7.78%, and individual variation was accounted for 28.14%. There was a rich genetic diversity among the populations of L. brandtii except Zhengxiangbaiqi population. The majority of genetic variation occurred among groups and within populations.
2016,35(6): 818-824 收稿日期:2016-06-06
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