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Issue:ISSN 1000-7083
          CN 51-1193/Q
Director:Sichuan Association for Science and Technology
Sponsored by:Sichuan Society of Zoologists; Chengdu Giant Panda Breeding Research Foundation; Sichuan Association of Wildlife Conservation; Sichuan University
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Your Position :Home->Past Journals Catalog->2016 Vol.35 No.5

Mechanism of the Enhancement of Scorpion Venom from Heterometrus liangi on Apoptosis and Necrosis of KYSE-510 Cell
Author of the article:WANG Juan1#, LI Qinjin1,2#, FU Wenhui1, WEI Baoyang1, WANG Zhi1*
Author's Workplace:1. College of Bioscience & Biotechnology, Hunan Agriculture University, Changsha 410128, China;
2. Hunan Sports Vocational College, Changsha 410019, China
Key Words:venom of Heterometrus liangi; KYSE-510 cells; caspase-3; gene expression
Abstract:Scorpion venom was extracted from Heterometrus liangi to study the mechanism of inhibition of venom on tumor grow. The effect of scorpion venom on apoptosis and necrosis of KYSE-510 cell and expression levels of caspase-3 were investigated by electron microscopy, Annexin V-FITC, and propidiumiodide double staining analysis by flowcytometry as well as reverse transcription PCR and Western blot. The results indicated that the mRNA expression levels of caspase-3 in KYSE-510 cells that treated with different concentrations of scorpion venom (50 μg·mL-1, 100 μg·mL-1, 200 μg·mL-1) were similar to these of control. However, the expressions of caspase-3 in KYSE-510 cells were obviously up-regulated by scorpion venom as determined by Western blot, especially when the cells were treated with 200 μg·mL-1 scorpion venom. These results suggested that the possible mechanism of the scorpion venom was accelerated protein splicing activity of caspase-3.
2016,35(5): 660-665 收稿日期:2016-06-03
DOI:10.11984/j.issn.1000-7083.20160152
分类号:Q78
基金项目:湖南省科技厅重点项目(2014FJ2003)
作者简介:王娟(1990-),女,硕士研究生,研究方向为发育生态学,E-mail:240647109@qq.com;李秦晋(1975-),男,博士研究生,讲师,主要从事农业生态学研究,E-mail:18157099@qq.com
*通讯作者:王智,博士,教授,博士研究生导师,主要从事生态学和害虫生态调控研究,E-mail:wangzspider@sina.com.cn
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