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Issue:ISSN 1000-7083
          CN 51-1193/Q
Director:Sichuan Association for Science and Technology
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Your Position :Home->Past Journals Catalog->2016 Vol.35 No.5

Comparison of the mRNA Expression Level of ERK1, ERK2 and P38 Genes in the Testes of Yaks and Sterile Cattle-yaks
Author of the article:LEI Jiewen, HUANG Lin, JIN Suyu, ZHENG Yucai*
Author's Workplace:College of Life Science and Technology, Southwest University for Nationalities, Chengdu 610041, China
Key Words:yak; cattle-yak; MAPK signaling pathway; ERK1; ERK2; P38
Abstract:MAPK signaling pathway plays an important role in the regulation of spermatogenesis and sperm apoptosis in mammals, and it is considered to be one of the important determinants of spermatogenesis. To explore the molecular mechanism of male sterility of cattle-yaks, the expression levels of ERK1, ERK2 and P38 genes [the mitogen-activated protein kinase (MAPK) pathway] in the testes of yaks and cattle-yaks were compared in this study. Total RNA was extracted from the testes of yaks (n=10) and cattle-yaks (n=7). The expression levels of ERK1, ERK2 and P38 genes in the testes of yaks and cattle-yaks were examined by real-time fluorescence quantitative PCR. The mRNA expression levels of ERK1 and ERK2 genes in the testes of yaks were very significantly higher than those of cattle-yaks (P<0.01). Moreover, although the expression of P38 gene was detected in both testes of yaks and cattle-yaks, no significant difference was found. The results of this study suggested that ERK1 and ERK2 may be associated with the abnormal spermatogenesis in cattle-yak testes through MAPK signaling pathway.
2016,35(5): 666-671 收稿日期:2016-05-02
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