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Issue:ISSN 1000-7083
          CN 51-1193/Q
Director:Sichuan Association for Science and Technology
Sponsored by:Sichuan Society of Zoologists; Chengdu Giant Panda Breeding Research Foundation; Sichuan Association of Wildlife Conservation; Sichuan University
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Your Position :Home->Past Journals Catalog->2016 Vol.35 No.4

Study on the Morphology and Ultrastructure of Hucho bleekeri Kimura Spermatozoa
Author of the article:GUO Wei1,2,3, LI Ping3, YANG Huanchao3,4, DU Hao3, WU Jinming3, WEI Qiwei1,3,4*
Author's Workplace:1. Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China;
2. University of Chinese Academy of Sciences, Beijing 100039, China;
3. Key Laboratory of Freshwater Biodiversity Conservation, Ministry of Agriculture of China, Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan 430223, China;
4. Huazhong Agricultural University, Wuhan 430070, China
Key Words:Hucho bleekeri Kimura; spermatozoa; ultrastructure
Abstract:This study was conducted to investigate the morphology and ultrastructure of Hucho bleekeri Kimura spermatozoa through scanning and transmission electron microscopy. The results revealed that the spermatozoa can be differentiated into three major parts: a head, a mid-piece, and a flagellum. The mean length of the spermatozoa was 41.07 μm±2.18 μm, with a spherical head length of 2.76 μm±0.15 μm and a flagellum length of 34.74 μm±5.01 μm. The mean anterior and posterior head widths were 1.88 μm±0.18 μm and 2.08 μm±0.20 μm, respectively. The head was spherical without acrosome, which was composed of nucleus. The short mid-piece was located laterally to the nucleus and possessed just one spherical mitochondrion with a mean diameter of 0.82 μm±0.08 μm. The spermatozoa flagellum was long and cylindrical, and could be separated into two parts. The axoneme composed the typical "9+2" microtubular doublet structure and was enclosed by the cell membrane. The flagellum had short irregular side-fins. This study confirmed that H. bleekeri Kimura spermatozoa can be categorized as teleostean Type Ⅰ; "primitive" or "ect-aquasperm type" spermatozoa.
2016,35(4): 550-555 收稿日期:2016-03-07
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