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Issue:ISSN 1000-7083
          CN 51-1193/Q
Director:Sichuan Association for Science and Technology
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Your Position :Home->Past Journals Catalog->2016 Vol.35 No.4

Expression Profiles of Genes Encoding Temperature-Sensitive Ion Channels in Mammalian Internal Organs and Blood Vessels
Author of the article:LIU Haoran, FAN Xinrong, LI Pengyun, LI Tao, ZENG Bo*
Author's Workplace:Key Laboratory of Medical Electrophysiology, Ministry of Education, Insitute of Cardiovascular Research, Southwest Medical University, Luzhou, Sichuan Province 646000, China
Key Words:temperature-sensitive; transient receptor potential channels; mammal; gene expression; internal organ; blood vessel
Abstract:Temperature-sensitive ion channels widely exist in internal organs and blood vessels of mammals and play important roles in the regulation of various physiological functions. In this study, we collected tissues including the heart, liver, spleen, kidney, lung, aorta, pulmonary artery/vein, femoral artery/vein, and carotid artery from dogs and rats, respectively. Subsequently, the expression of genes encoding temperature-sensitive ion channels were detected using reverse transcription PCR and agarose gel electrophoresis. The results showed that TRPV1 existed in all the tissues of the two species examined, while TRPV2 and TRPM5 were undetectable in all the examined tissues. TRPV3 was expressed in the liver, lung and pulmonary artery of rat but absent in all canine tissues. TRPV4 existed in all the tissues of both species except in the lung of dog. TRPM2 was expressed in all the tissues of both species except in the aorta, heart and pulmonary artery of rat. TRPM3 expression was absent in the lung and pulmonary artery of dog and the lung, heart and aorta of rat, but presented in all other tissues. TRPM4 existed in all the tissues of both species except the pulmonary artery of dog and spleen, kidney and heart of rat. TRPM8 existed in all the tissues of both species except the liver, spleen and kidney of dog and kidney of rat. TRPA1 expressoin was found only in the lung and spleen of rat. These results provided detailed information for the expression of genes encoding temperature-sensitive ion channels in mammalian internal organs and blood vessels, which were essential for further exploration of physiological regulation by these channels.
2016,35(4): 517-522 收稿日期:2016-03-14
DOI:10.11984/j.issn.1000-7083.20160052
分类号:Q24;Q959.8
作者简介:刘浩然(1994-),男,本科生,E-mail:oromis@foxmail.com
*通讯作者:曾博,E-mail:zengbo@swmu.edu.cn
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