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Issue:ISSN 1000-7083
          CN 51-1193/Q
Director:Sichuan Association for Science and Technology
Sponsored by:Sichuan Society of Zoologists; Chengdu Giant Panda Breeding Research Foundation; Sichuan Association of Wildlife Conservation; Sichuan University
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Your Position :Home->Past Journals Catalog->2016 Vol.35 No.4

Molecular Cloning and Tissue Distribution of Kisspeptin from Sinocyclocheilus tingi
Author of the article:LIAO Zongzhen1, SUN Caiyun1, WANG Xiaoai2, YANG Guokun1, PAN Xiaofu2, YANG Junxing2*, LI Wensheng1*
Author's Workplace:1. State Key Laboratory of Biocontrol, Institute of Aquatic Economic Animals and Guangdong Province Key Laboratory for Aquatic Economic Animals, Research Institute of Sun Yat-Sen University in Shenzhen, School of Life Sciences, Sun Yat-Sen University, Guangzhou 510275, China;
2. State Key Laboratory of Genetic Resources and Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, China
Key Words:Sinocyclocheilus tingi; kisspeptin; kiss1; kiss2; clone; characteristics of tissue distribution
Abstract:Sinocyclocheilus tingi is an endemic species of Fuxian Lake in Yunnan province. Even though its artificial reproduction is available right now, they can't reproduce in fishponds. Kisspeptin encoded by kiss1 was considered as an important regulator of the hypothalamic-pituitary-gonadal axis and took part in reproduction by controlling the releasing of gonadotropin-releasing hormone, gonadotropin, and etc. In the present research we cloned the cDNA of kiss1 and kiss2 of S. tingi with reverse transcription PCR and rapid amplification of cDNA ends (RACE). The open reading frame of kiss1 was 351 bp and encoded a polypeptide of 116 amino acid and that of kiss2 was 369 bp and encoded a 122-amino-acid polypeptide. Reverse transcription PCR analysis showed that the mRNA expression quantity of kiss1 was the highest in intestine. Likely, the mRNA expression of kiss2 was the highest in intestine in male and hypothalamus and brain in female. To explore the distribution of kiss1 and kiss2 in brain, the brain was divided into eight parts for reverse transcription PCR analysis. The result showed that the highest expression of kiss1 in male was observed in both the spinal cord and the pituitary, which in female was only in the pituitary and followed by the optic tectum. Meanwhile, the kiss2 mRNA got its highest expression in the hypothalamus.
2016,35(4): 496-502 收稿日期:2015-09-08
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