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Issue:ISSN 1000-7083
          CN 51-1193/Q
Director:Sichuan Association for Science and Technology
Sponsored by:Sichuan Society of Zoologists; Chengdu Giant Panda Breeding Research Foundation; Sichuan Association of Wildlife Conservation; Sichuan University
Address:College of Life Sciences, Sichuan University, No.29, Wangjiang Road, Chengdu, Sichuan Province, 610064, China
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Your Position :Home->Past Journals Catalog->2016 Vol.35 No.4

Effect of Exposure Time of Ailuropoda melanoleuca Feces on Microsatellites Analysis
Author of the article:WANG Chengdong1, YANG Bo1, HUANG Jie2,3, HUANG Yan1, CHENG Yanxi1, LI Rengui1, HUANG Shan1, LI Desheng1, ZHANG H
Author's Workplace:1. China Conservation and Research Center for the Giant Panda, Wolong, Sichuan Province 623006, China;
2. Sichuan Key Laboratory of Conservation Biology on Endangered Wildlife, College of Life Sciences, Sichuan University, Chengdu 610064, China;
3. College of Life Sciences, Shangqiu Normal University, Shangqiu, Henan Province 476000, China
Key Words:Ailuropoda melanoleuca; feces; exposure time; microsatellite
Abstract:Noninvasive sampling methods, such as acquiring host genomic DNA from fecal samples for molecular biology research, are increasingly used in endangered wild animals. However, it is difficult to obtain fresh feces in the field. By focusing on the effects of feces after different exposure times in the field on molecular studies for giant pandas (Ailuropoda melanoleuca), we found that shorter exposure time of feces would lower the level of DNA degradation, and increase the quality of the feces DNA. The degradation level of feces DNA was also related to seasonal variation and field environment. The valid time was no longer than 14 days in spring and autumn, less than 7 days in summer and no longer than 21 days in winter. Moreover, dry environment such as the entrance of the cave may be the best place for preserving feces samples in the field. The results of this study provided guidance for the feces collection of wild giant pandas in the field.
2016,35(4): 481-487 收稿日期:2016-02-16
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