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Issue:ISSN 1000-7083
          CN 51-1193/Q
Director:Sichuan Association for Science and Technology
Sponsored by:Sichuan Society of Zoologists; Chengdu Giant Panda Breeding Research Foundation; Sichuan Association of Wildlife Conservation; Sichuan University
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Your Position :Home->Past Journals Catalog->2016 Vol.35 No.3

Expression of Glucocorticoid Receptors and Mineralocorticoid Receptors in the Brain of Passer montanus after Cadmium Treatment
Author of the article:SUO Lijuan1, LUO Lei1, GAO Xuebin1, ZHAO Hongfeng2*
Author's Workplace:1. Shaanxi Institute of Zoology, Xi'an 710032, China;
2. College of Life Science, Shaanxi Normal University, Xi'an 710119, China
Key Words:glucocorticoid receptors; mineralocorticoid receptors; Passer montanus; gene expression; stress
Abstract:Objective The expression levels of glucocorticoid receptors (GR) and mineralocorticoid receptors (MR) were measured in the brain of Passer montanus after cadmium treatment. Method Six P. montanus were randomly divided into 2 groups. Treatment group was fed with water of 500μg·L-1 Cd2+. Alignment of amino acid sequence of GR and MR was performed using the ClustalW2. Real-time PCR was performed to study the baseline expression levels of GR and MR among different tissues. Finally, the expressions of GR and MR were determined in the brain of P. montanus after cadmium treatment. Result The result of amino acid sequence alignment showed that the GR and MR of P. montanus had high similarity with Taeniopygia guttata and Gallus gallus (>90%). Real-time PCR results showed that the GR and MR expressed in all of nine detected organs and tissues. Compared with the control, the expression levels of GR and MR were significantly up-regulated after cadmium treatment (P<0.05). Conclusion The GR and MR were widely expressed in tissues of P. montanus. GR and MR might play a significant role under the cadmium stress. This research provided the experimental evidence for fieldwork.
2016,(35): 409-413 收稿日期:2015-11-05
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