Latest Cover

Online Office

Contact Us

Issue:ISSN 1000-7083
          CN 51-1193/Q
Director:Sichuan Association for Science and Technology
Sponsored by:Sichuan Society of Zoologists; Chengdu Giant Panda Breeding Research Foundation; Sichuan Association of Wildlife Conservation; Sichuan University
Address:College of Life Sciences, Sichuan University, No.29, Wangjiang Road, Chengdu, Sichuan Province, 610064, China
Fax:+86-28-85410485 &
Your Position :Home->Past Journals Catalog->2016 Vol.35 No.3

Redescription of Myxidium cuneiforme Fujita, 1924 (Myxosporea: Bivalvulida) and Molecular Phylogeny with its Relative Species
Author of the article:LI Chao, SUO Dong, YANG Chengzhong*, ZHAO Yuanjun
Author's Workplace:Chongqing Key Laboratory of Animal Biology, College of Life Sciences, Chongqing Normal University, Chongqing 401331, China
Key Words:Myxidium cuneiforme; relative species; 18S rDNA; molecular phylogeny
Abstract:The species identification between Myxidium and Zschokklella is difficult due to their similar morphology and vague boundaries. To clarify the taxonomic relationship of the two genera, the morphology of Myxidium cuneiforme Fujita, 1924 collected from the Fujiang River in Tongnan county, Chongqing, China was redescribed. Subsequently, molecular phylogeny was analyzed along with the relative species. The mature spore of M. cuneiforme was elongated in valvular view with the middle part concaved slightly. The surface of each valve had 6~8 longitudinal grooves. The suture line was straight, and spore was fusiform in sutural view. The spore was 12.5 μm±0.3 μm long and 5.4 μm±0.3 μm wide (n=20), each with two polar capsules posited at the both end of the spore. The two polar capsules were pyriform with equal size of 4.3 μm±0.2 μm long and 3.1 μm±0.2 μm wide (n=20), containing polar filaments coiled 5~6 turns. Analyses of the variation of conserved regions, genetic distances, sequence similarities and molecular phylogeny based on 18S rDNA sequences indicated that, Myxidium and Zschokkella were not monophyletic, as they clustered together with each other in the phylogenetic analysis. Therefore, Myxidium and Zschokkella had a very close genetic relationship. The results from the morphological and molecular data supported the proposal that Myxidium and Zschokkella should be assigned to one single genus.
2016,(35): 384-390 收稿日期:2015-11-06
陈启鎏, 马成伦. 1998. 中国动物志 粘体门 粘孢子纲(淡水)[M]. 北京:科学出版社:1-30.
鲁义善, 聂品. 2004. 淡水鱼类粘孢子虫18S rDNA分子系统学研究[J]. 水生生物学报, 28(6):587-591.
冉佼, 杨承忠, 赵元莙. 2014. 基于遗传距离的粘孢子虫分类研究[J]. 重庆师范大学学报(自然科学版), 31(3):31-34.
宋微波, 赵元莙, 徐奎栋, 等. 2003. 海水养殖中的危害性原生动物[M]. 北京:科学出版社:315-428.
张婧, 莫西, 李楠楠, 等. 2015a. 徐家汇尾孢虫Henneguya zikawiensis Sikama, 1938补充描述及其分子系统学研究[J]. 内江师范学院学报, 30(4):42-46.
张婧, 杨承忠, 赵元莙. 2015b. 碘泡科(粘体门:粘孢子纲:双壳目)动物的系统分类学研究[J]. 四川动物, 34(3):474-480.
赵元莙, 马成伦, 宋微波. 2001. 海水养殖中原生动物病原虫的鉴定-Ⅱ 粘孢子虫原生动物的研究及鉴定方法[J]. 青岛海洋大学学报(自然科学版), 31(5):681-688.
钟霞, 毛华明, 邓卫东. 2007. 18S rRNA/rDNA序列分析技术在瘤胃原虫分类学研究中的应用[J]. 中国畜牧兽医, 34(12):56-58.
Barta JR, Martin DS, Libertor PA, et al. 1997. Phylogenetic relationships among eight Eimeria species infecting domestic fowl inferred using complete small subunit ribosomal DNA sequences[J]. The Journal of Parasitology, 83(2):262-271.
Bartošová P, Fiala I, Hypša V. 2009. Concatenated SSU and LSU rDNA data confirm the main evolutionary trends within myxosporeans (Myxozoa:Myxosporea) and provide an effective tool for their molecular phylogenetics[J]. Molecular Phylogenetics and Evolution, 53(1):81-93.
Canning EU, Okamura B. 2004. Biodiversity and evolution of the myxozoa[J]. Advances in Parasitology, 56:44-131.
Diamant A, Lom J, Dyková I. 1994. Myxidium leei n. sp., a pathogenic myxosporean of cultured sea bream Sparus aurata[J]. Diseases of Aquatic Organisms, 20(2):137-141.
Dyková I, Tyml T, Fiala I, et al. 2007. New data on Soricimyxum fegati (Myxozoa) including analysis of its phylogenetic position inferred from the SSU rRNA gene sequence[J]. Folia Parasitologica, 54(4):272-276.
Eiras JC, Saraiva A, Santos MJ, et al. 2011. Synopsis of the species of Myxidium Bütschli, 1882 (Myxozoa:Myxosporea:Bivalvulida)[J]. Systematics Parasitology, 80:81-116.
Fiala I. 2006. The phylogeny of Myxosporea (Myxozoa) based on small subunit ribosomal RNA gene analysis[J]. International Journal for Parasitology, 36(14):1521-1534.
Fujita T. 1924. Studies on myxosporidian infection of the crucian carp[J]. Japanese Journal of Zoology, 1:45-57.
Kent ML, Andree KB, Bartholomew JL, et al. 2001. Recent advances in our knowledge of the Myxozoa[J]. The Journal of General and Applied Microbiology, 48(4):395-413.
Lom J, Dyková I. 2006. Myxozoan genera:definition and notes on taxonomy, life cycle terminology and pathogenic species[J]. Folia Parasitologica, 53:1-36.
Michel R, Walochnik J, Aspöck H. 2003. Pseudodidymium cryptomastigophorum gen. n., sp. n., a Hyperamoeba or a slime mould? A combined study on morphology and 18S rDNA sequence data[J]. Acta Protozoologica, 42(4):331-342.
Michielsen NC. 1966. Intraspecific and interspecific competition in the shrews Sorex araneus L. and S. minutus L.[J]. Archives Neerlandaises de Zoologie, 17(1):73-174.
Prunescu CC, Prunescu P, Pucek Z, et al. 2007. The first finding of myxosporean development from plasmodia to spores in terrestrial mammals:Soricimyxum fegati gen. et sp. n. (Myxozoa) from Sorex araneus (Soricomorpha)[J]. Folia Parasitologica, 54:159-164.
Ronquist F, Huelsenbeck JP. 2003. MrBayes 3:Bayesian phylogenetic inference under mixed models[J]. Bioinformatics, 9:1572-1574.
Swofford DL. 2001. PAUP:phylogenetic analysis using Parsimony (and other methods) version 4[M]. Sunderland, Massachusetts:Sinauer Associates.
Tamura K, Stecher G, Peterson D, et al. 2013. MEGA6:molecular evolutionary genetics analysis version 6.0[J]. Molecular Biology and Evolution, 30:2725-2729.
Whipps CM, Zhao YJ. 2015. Synopsis of the species of the genus Sphaeromyxa Thélohan, 1892 (Myxosporea:Bivalvulida:Variisporina Sphaeromyxidae[J]. Systematics Parasitology, 92(2):81-99.
Wright ADG, Lynn DH. 1997. Phylogenetic analysis of the rumen ciliate family Ophryoscolecidae based on 18S ribosomal RNA sequences, with new sequences from Diplodinium, Eudiplodinium, and Ophryoscolex[J]. Canadian Journal of Zoology, 75(6):963-970.
Zhao YJ, Li NN, Tang FH, et al. 2013. Remarks on the validity of Myxobolus ampullicapsulatus and Myxobolus honghuensis (Myxozoa:Myxosporea) based on SSU rDNA sequences[J]. Parasitology Research, 112(11):3817-3823.
CopyRight©2020 Editorial Office of Sichuan Journal of Zoology