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Issue:ISSN 1000-7083
          CN 51-1193/Q
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Your Position :Home->Past Journals Catalog->2016 Vol.35 No.3

Cloning and Expression of Cytochrome P450 CYP6J1 from Aphis gossypii
Author of the article:WANG Yamei1, HUANG Lina1, AI Xinyu1, WEI Yuanjie1, LIU Xiaoning1*, GAO Xiwu2*
Author's Workplace:1. College of Life Sciences and Technology, Xinjiang University, Xinjiang Key Laboratory of Biological Resources and Genetic Engineering, Urumqi 830046, China;
2. College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, China
Key Words:Aphis gossypii; P450 CYP6J1; cloning; protein expression
Abstract:In order to understand the structure and function of cytochrome P450 CYP6J1 protein, P450CYP6J1 gene from cotton aphid (Aphis gossypii) was cloned and analyzed using bioinformatic method. P450 CYP6J1 protein was first expressed in prokaryotic, followed by SDS-PAGE and Western-blot detection. The results showed that the length of open reading frame region was 1398 bp, encoding 465 amino acids residues with the predicted molecular weight of 53.67 kD and isoelectric point of 8.80. Amino acid sequence analysis showed that P450 CYP6J1 protein had no signal peptide. Homology analysis showed that the amino acids of P450 CYP6J1 of A. gossypii was similar with that of pea aphid (Acyrthosiphon pisum) (homologous similarity 92%). The His-CYP6J1 protein was obtained after being expressed in Escherichia coli BL21, and the target protein was proved by Western-blot. These results provided a foundation for preparation of P450 CYP6J1 polyclonal antibody from A. gossypii.
2016,(35): 378-383 收稿日期:2015-12-08
DOI:10.11984/j.issn.1000-7083.20150393
分类号:Q78;Q969.35
基金项目:国家自然科学基金项目(31330064,31471781)
作者简介:王亚美(1990-),硕士研究生,研究方向:生化与分子生物学,E-mail:wangyamei0114@sina.com
*通讯作者:刘小宁,博士,研究方向:农业昆虫与害虫防治,E-mail:liuxn0103@sina.com;高希武,gaoxiwu@263.net.cn
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