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Issue:ISSN 1000-7083
          CN 51-1193/Q
Director:Sichuan Association for Science and Technology
Sponsored by:Sichuan Society of Zoologists; Chengdu Giant Panda Breeding Research Foundation; Sichuan Association of Wildlife Conservation; Sichuan University
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Your Position :Home->Past Journals Catalog->2015 Vol.34 No.2

The Effect of Food Water Deprivation on the Activity of Mitochondrial Respiratory Chain Complexes and Correlated Free Radical Metabolism in the Kidney and Liver Tissues of Meriones meridianus
Author of the article:DU Haidi, HOU Tiande*, CAO Wangjie, CHENG Fang, SANG Qiu, ZONG Hui
Author's Workplace:College of Life Sciences, Northwest Normal University, Lanzhou 730070, China
Key Words:food water deprivation; Meriones meridianus; mitochondria; the activity of respiratory chain complexesⅠ-Ⅳ; kidney and liver tissues
Abstract:Objective To observe the effect of food water deprivation on the enzyme activity of mitochondrial respiratory chain complexes in kidney and liver tissues and investigate the reactive characteristics of the mitochondrial energy metabolism. Methods The adult healthy Meriones meridianus was used as animal model and the change of enzyme activity of mitochondrial respiratory chain complex, the activity of superoxide dismutase (SOD) and the content of malondialdehyde (MDA) in kidney and liver tissues were determined with spectrophotometric method after water restriction of 3, 6 and 9 days, respectively. Results Food water deprivation can significantly increase the activity of four mitochondrial respiratory chain complexes in kidney and liver tissues (P<0.05, P<0.01), but gradually reduce the body weight. Three days post treatment was an important stage of adaptation reaction during the process of food water deprivation. The activity of respiratory chain complex increased most significantly after 3 days and all of them were reduced at Day 9, but were still higher than that of control. The activity of SOD in kidney mitochondria increased to varying degrees, and the content of MDA in kidney and liver mitochondria were significantly increased during water deprivation. Conclusion Food water deprivation can significantly increase the activity of mitochondrial respiratory chain complexes in kidney and liver tissues, and this may relate to the raise of water reabsorption in kidney and metabolism in liver. Long term water deprivation induced free radical level increase may have a negative effect on the maintenance of metabolism enzyme activity.
2015,34(2): 276-280 收稿日期:2014-7-4
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