Latest Cover

Online Office

Contact Us

Issue:ISSN 1000-7083
          CN 51-1193/Q
Director:Sichuan Association for Science and Technology
Sponsored by:Sichuan Society of Zoologists; Chengdu Giant Panda Breeding Research Foundation; Sichuan Association of Wildlife Conservation; Sichuan University
Address:College of Life Sciences, Sichuan University, No.29, Wangjiang Road, Chengdu, Sichuan Province, 610064, China
Tel:+86-28-85410485
Fax:+86-28-85410485
Email:scdwzz@vip.163.com & scdwzz001@163.com
Your Position :Home->Past Journals Catalog->2015 Vol.34 No.2

Effects of Dietary Copper Levels on Expression of Prophenoloxidase System-related Genes in Haemocyte of Penaeus monodon
Author of the article:XIAN Jianan1,2, WANG Anli2, MIAO Yutao2, QIAN Kun2, WANG Dongmei1
Author's Workplace:1. Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, China;
2. Key Laboratory of Ecology and Environmental Science of Guangdong Higher Education Institutes, Guangdong Provincial Key Laboratory for Healthy and Safe Aquaculture, College of Life Sciences, South China Normal University, Guangzhou 510631, China
Key Words:copper; Penaeus monodon; prophenoloxidase system; gene expression
Abstract:An 8-week feeding trial was conducted to investigate the effects of different concentrations of dietary copper (0, 10, 25, 40, 55 and 110 mg supplemental Cu/kg diet) on the expression of prophenoloxidase system-related genes (proPO1, proPO2, PE, PPAF and BGBP) in tiger shrimp Penaeus monodon haemocyte. The results showed that, compared to the control, expression of proPO1 increased significantly in shrimp fed with diets containing 40-55 mg·kg-1 of Cu. Expression levels of proPO2, PE and PPAF increased significantly in shrimp fed with diets containing 25-55 mg·kg-1 of Cu. Cu supplementation did not affect the expression of BGBP. Serum PO activity also increased in shrimp fed with diets supplemented with 25-55 mg·kg-1 of Cu. These results indicated that adequate supplemental Cu would induce the gene expression of proPO1, proPO2, PE and PPAF, and then improve the serum PO activity. In conclusion, for the improvement of prophenoloxidase system, the adequate supplemental Cu concentration was 25-55 mg·kg-1 for the present basal diet.
2015,34(2): 270-275 收稿日期:2014-8-1
DOI:10.3969/j.issn.1000-7083.2015.02.018
分类号:S963
基金项目:国家自然科学基金项目(31302164,30671628);广东省自然科学基金项目(S2011020003256,S2012040008093)
作者简介:冼健安(1983—),男,博士,助理研究员,主要研究方向:水产动物营养与饲料学,水产养殖生态及毒理学,E-mail:xian-ja@163.com
*通讯作者:王冬梅,女,研究员,主要研究方向:水产动物营养与饲料学,水产养殖生态及毒理学,E-mail:wdmei1969@126.com
参考文献:
董晓慧, 杨原志, 郑石轩, 等. 2007. 饲料中不同铜源和水平对凡纳滨对虾生长、免疫和组织铜含量的影响[J]. 大连水产学院学报, 22(5): 377-383.
郭慧, 冼健安, 毕建柱, 等. 2013. 虾类免疫因子的研究进展[J]. 饲料工业, 34(22): 42-46.
郭腾飞, 黄旭雄, 苏明, 等. 2012. 饲料中铜水平对凡纳滨对虾免疫相关基因表达和抗菌能力的影响[J]. 水生生物学报, 36(5): 809-816.
郭志勋, 陈毕生, 徐力文, 等. 2003. 饲料铜的添加量对南美白对虾生长、血液免疫因子及组织铜的影响[J]. 中国水产科学, 10(6): 526-528.
刘发义, 梁德海, 孙凤, 等. 1990. 饵料中的铜对中国对虾的影响[J]. 海洋与湖沼, 21(5): 404-410.
冼健安, 苗玉涛, 叶超霞, 等. 2013. 饲料铜含量对亚硝酸盐胁迫下斑节对虾血细胞的影响[J]. 水生态学杂志, 34(5): 71-75.
冼健安, 王安利. 2013. 饲料中铜离子对斑节对虾血细胞组成、活性和免疫功能的影响[J]. 海洋科学, 37(2): 40-46.
阳会军, 谭北平, 方怀义. 2001. 饲料中添加蛋氨酸铜和硫酸铜对斑节对虾生长和存话的影响[J]. 饲料工业, 22(10): 15-16.
周萌, 王安利, 曹俊明. 2010. 饲料中不同形式的铜及添加量对凡纳滨对虾(Paneaus vannamei)生长性能、血清铜蓝蛋白和生长激素水平的影响[J]. 海洋与湖沼, 41(4): 577-582.
Huang JW, Yang Y, Wang AL. 2010. Reconsideration of phenoloxidase activity determination in white shrimp Litopenaeus vannamei[J]. Fish & Shellfish Immunology, 28: 240-244.
Lee MH, Shiau SY. 2002. Dietary copper requirement of juvenile grass shrimp, Penaeus monodon, and effects on non-specific immune responses[J]. Fish & Shellfish Immunology, 13: 259-270.
Liu CH, Yeh SP, Kuo CM, et al. 2006. The effect of sodium alginate on the immune response of tiger shrimp via dietary administration: Activity and gene transcription[J]. Fish & Shellfish Immunology, 21: 442-452.
Wang YC, Chang PS, Chen HY. 2008. Differential time-series expression of immune-related genes of Pacific white shrimp Litopenaeus vannamei in response to dietary inclusion of β-1,3-glucan[J]. Fish & Shellfish Immunology, 24: 113-121.
CopyRight©2019 Editorial Office of Sichuan Journal of Zoology