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Issue:ISSN 1000-7083
          CN 51-1193/Q
Director:Sichuan Association for Science and Technology
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Your Position :Home->Past Journals Catalog->2015 Vol.34 No.1

Influence of the Ovary Size to Oocyte in vitro Mature in Goat
Author of the article:YU Honghao1,2, YAN Hailong1,2, ZHANG Yonghua1,2, JING Xiaoqi1,2, FENG Ping1,2, QU Lei1,2*
Author's Workplace:1. Life Science Research Center, Yulin University, Yulin, Shaanxi Province 719000, China;
2. Shaanxi Cashmere Goat Engineering and Technology Center, Yulin, Shaanxi Province 719000, China
Key Words:goat; ovary; oocyte; in vitro mature
Abstract:Objective The influence of ovary size on the in vitro maturity of oocyte was investigated. Method The ovaries of goat from slaughter house were divided into three groups according to the weight (groupⅠ<0.95 g; group Ⅱ, 0.95~1.7 g; group Ⅲ>1.7 g). Some statistic indices including maturation rate, parthenogenetic cleavage rate, parthenogenetic blastocyst rate, cloned embryo cleavage rate and cloned embryo blastocyst rate of the three groups were determined in vitro. Result The in vitro maturation rates of oocytes from different groups were 62.25%, 43.58% and 40.7%, respectively. Parthenogenetic cleavage rates of oocytes from different groups were 83.77%, 82.92% and 79.73%, respectively. Parthenogenetic blastocyst rates of oocytes from different groups were 19.51%, 18.9% and 18.78%, respectively. Cleavage rates of cloned embryo reconstructed using oocytes from the three groups by handmade cloning method were 67.36%, 65.97% and 66.33%, respectively, and the blastocyst rates of the cloned embryos were 11.63%, 13.41% and 12.29%, respectively. Oocyte maturation rate of groupⅠwas significantly higher than that of others. Moreover, no significant difference of parthenogenetic cleavage rate, parthenogenetic blastocyst rate, cleavage rate of cloned embryos and blastocyst rate of cloned embryos was observed among the three. Conclusion The results showed that the in vitro maturation rate of oocytes of small size ovaries was higher than that of the big ones. The size of ovary only affects oocyte maturation rate rather than developmental capacity.
2015,34(1): 84-87 收稿日期:2014-4-17
DOI:10.3969/j.issn.1000-7083.2015.01.014
分类号:Q959.8;Q81
基金项目:陕西省科技厅农业科技攻关项目(2011K01-05);陕西省科技厅自然科学基础项目(2013JM3020);榆林学院高层次人才启动金项目(11GK05)
作者简介:于鸿浩,男,博士,副教授,主要从事转基因动物研究,E-mail:geneyhh@126.com
*通讯作者:屈雷,E-mail:ylqulei@126.com
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