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Issue:ISSN 1000-7083
          CN 51-1193/Q
Director:Sichuan Association for Science and Technology
Sponsored by:Sichuan Society of Zoologists; Chengdu Giant Panda Breeding Research Foundation; Sichuan Association of Wildlife Conservation; Sichuan University
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Your Position :Home->Past Journals Catalog->2015 Vol.34 No.4

Antimicrobial Resistance and Integrons of Escherichia coli Isolates from Giant Pandas Feces
Author of the article:YAN Guodong, LIU Songrui, HOU Rong, GAO Tongtong, WANG Shuang, DAI Pengfei, DENG Lin, LIN Juchun
Author's Workplace:1. College of Veterinary Medicine, Sichuan Agricultural University, Ya'an, Sichuan Province, 625014, China;
2. Chengdu Research Base of Giant Panda Breeding, Chengdu 610081, China
Key Words:giant panda; fecal Escherichia coli; resistance; integron-gene cassette
Abstract:To investigate the antimicrobial resistance, distribution of integron-gene cassettes of Escherichia coli that isolated from the feces of giant pandas, as well as determine the correlation between resistance and integron-gene cassette, Kirby-Bauer method was used to test the susceptibility of 50 E. coli isolates against 13 antimicrobial agents. PCR-sequencing was established to detect the integrons (class 1, 2, 3) and gene cassettes that were harbored in E. coli isolates. The results showed that the isolates had different resistant levels to the 13 tested antimicrobials: high resistant level was observed in ampicillin, cefazolin, tetracycline and SMZ-TMP (resistant rates ranged from 30% to 68%); 30% of the isolates were found to harbor the class 1 integron, and no class 2 or class 3 integron was detected; gene cassettes with length of 1200-2000 bp were amplified from 40% of the 15 intI1-positive isolates, and the main resistant gens were identified as encoding aminoglycoside adenyltransferase (aadA) and dihydrofolate reductase (dfrA). The main arrays of gene cassettes were dfrA27+aadA2 (detection rate was 83.33%), moreover, 1 isolate was found to harbor the gene cassettes aacA4+aadA1+catB2 discovered. In conclusion, the E. coli isolates from the feces of giant pandas were sensitive to majority of the tested antimicrobial agents. Class l integron was widely distributed in E. coli strains from giant pandas, and integron-gene cassettes were responsible for resistant to aminoglycosides, sulfonamides-TMP and chloramphenicol.
2015,34(4): 489-493 收稿日期:2014-11-22
DOI:10.11984/j.issn.1000-7083.2015.04.002
分类号:Q93
基金项目:国家科技支撑计划项目(2012BAC01B06)
作者简介:闫国栋(1988—),男,硕士研究生,主要从事细菌耐药性及耐药机制研究,E-mail:guodong0973@163.com
*通讯作者:林居纯,教授,博士,硕士研究生导师,主要从事兽医药理及毒理学研究,E-mail:juchunlin@126.com
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