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Issue:ISSN 1000-7083
          CN 51-1193/Q
Director:Sichuan Association for Science and Technology
Sponsored by:Sichuan Society of Zoologists; Chengdu Giant Panda Breeding Research Foundation; Sichuan Association of Wildlife Conservation; Sichuan University
Address:College of Life Sciences, Sichuan University, No.29, Wangjiang Road, Chengdu, Sichuan Province, 610064, China
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Your Position :Home->Past Journals Catalog->2014 Vol.33 No.6

Comparative Analysis of Microsatellite Sequences Distribution in the Genome of Giant Panda and Polar Bear
Author of the article:LI Wujiao1#, LI Yuzhi1,2#, DU Lianming1, HUANG Jie1, SHEN Yongmei3, ZHANG Xiuyue1, YUE Bisong1*
Author's Workplace:(1. Sichuan Key Laboratory of Conservation Biology on Endangered Wildlife, College of Life Science, Sichuan University, Chengdu 610064, China; 2. Pharmacy College, Chengdu University of Traditional Chinese Medicine, Chengdu 610075, China; 3. Gooddoctor Pharmaceutical Group, Xichang, Sichuan Province 615000, China)
Key Words:giant panda; polar bear; genome; microsatellite sequences
Abstract: In this study, the distribution of microsatellite sequences in the whole genome of giant panda well as polar bear were analyzed and compared. A total of 855 018 and 936 238 perfect microsatellite sequences with 1~6 bp nucleotide motifs were identified from giant panda and polar bear, respectively. The total length of microsatellites was 14 919 240 bp (0.65% of the genome size) in giant panda, and 18 434 348 bp (0.79% of the genome size) in polar bear. The abundance of microsatellites was 405.6 no./Mbp in polar bear , and 371.81 no./Mbp in giant panda. Among of which, mononucleotide repeats (accounting for 48.56 %) was the most abundant, followed by the dinucleotide, tetranucleotide, trinucleotide, pentanucleotide and hexanucleotide repeats. A, AC, AG, AAAT, AAAG, AT and C were the most abundant repeat units shared by the two genomes. This study can provide a basis for developing and screening a large number of high-quality microsatellite markers for bear family.
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