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Issue:ISSN 1000-7083
          CN 51-1193/Q
Director:Sichuan Association for Science and Technology
Sponsored by:Sichuan Society of Zoologists; Chengdu Giant Panda Breeding Research Foundation; Sichuan Association of Wildlife Conservation; Sichuan University
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Your Position :Home->Past Journals Catalog->2014 Vol.33 No.4

Analysis of Forest Musk Deer (Moschus berezovskii) MHC-DRA Gene Exon 2 and Compared with Other Ruminantia
Author of the article:XIA Shan1,2, YUE Bisong1*
Author's Workplace:(1. Sichuan Key Laboratory of Conservation Biology on Endangered Wildlife, College of Life Sciences, Sichuan University, Chengdu 610064, China; 2. College of Chemistry and Life Science, Chengdu Normal University, Chengdu 611130, China)
Key Words:forest musk deer; MHC; DRA; Ruminantia
Abstract: The major histocompatibility complex (MHC) is one of the most important elements in immune system in almost all vertebrates. Mammals usually show low MHC-DRA polymorphism with the exception of horse, zebra and donkey. Cattle-specific primers DRA-F and DRA-R were used to amplify a 260 bp fragment of exon 2 of the DRA locus from genmonic DNA of 217 individuals of Chinese forest musk deer. We found 2 alleles on locus DRA and named them as Mobe-DRA*1 and Mobe-DRA*2. There was only one nucleotide differences (no amino acid differences) between the two DRA alleles. We downloaded 31 DRA sequences of Ruminantia from GenBank, and reconstructured the molecular phylogenetic relationship within Pecora using the Bayes Inference (BI). The phylogenetic relationships between these alleles supported the monophyly of Cervidae, Bovidae and Cetancodonta, and placed Moschidae as a sister group to Cervidae/Bovidae. The difference in 33 DRA sequences shows that changes in amino acid sequence fall outside the antigen binding site (ABS).
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