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Issue:ISSN 1000-7083
          CN 51-1193/Q
Director:Sichuan Association for Science and Technology
Sponsored by:Sichuan Society of Zoologists; Chengdu Giant Panda Breeding Research Foundation; Sichuan Association of Wildlife Conservation; Sichuan University
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Your Position :Home->Past Journals Catalog->2014 Vol.33 No.4

Molecular Cloning and Expression of SPI Gene in Cyclina sinensis
Author of the article:ZHANG Hao, ZHAO Ting, REN Yipeng, PAN Baoping*
Author's Workplace:(College of Life Sciences, Tianjin Key Laboratory of Animal and Plant Resistance, Tianjin Normal University, Tianjin 300387, China)
Key Words:Cyclina sinensis; Vibrio anguillarum; serine protease inhibitor; Kazal; realtime PCR
Abstract:The Kazal type serine protease inhibitor gene of Cyclina sinensis were acquired by constructing SMART-cDNA library and large scale EST sequencing method. The expression of the serine protease inhibitor gene in different tissues was determined by RT-PCR, additionally, the expression of which were also determined in hemocytes after injection of Vibrio anguillarum. The results showed that the full length of SPI is 587 bp, the CDS covers a region from 67 bp to 520 bp encoding a 151-amino acid peptide including a 19-amino acid signal peptide. Reverse transcription PCR analysis showed that the express of serine protease inhibitor gene in hemocytes, liver, gills, adducter and mantle have obvious difference, and the highest expression level was observed in hemocytes, followed by in adducter. The SPI expression in hemocytes was significantly increased (P<0.01) at 3 hours and 48 hours after the challenge of Vibrio anguillarum. Thus, SPI may play an important role in the immune response of Cyclina sinensis.
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