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Issue:ISSN 1000-7083
          CN 51-1193/Q
Director:Sichuan Association for Science and Technology
Sponsored by:Sichuan Society of Zoologists; Chengdu Giant Panda Breeding Research Foundation; Sichuan Association of Wildlife Conservation; Sichuan University
Address:College of Life Sciences, Sichuan University, No.29, Wangjiang Road, Chengdu, Sichuan Province, 610064, China
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Your Position :Home->Past Journals Catalog->2014 Vol.33 No.4

Effect of Supplemented FBS to the Maturation Medium on the External Mature Culturing of Oocyte from Goats
Author of the article:ZHU Guangxiang1,2*, MA Hengdong2,3, ZHANG Hua 4
Author's Workplace:(1. College of Life Sciences, Sichuan Agricultural University, Ya’an, Sichuan Province 625014, China; 2. College of Animal Science and Technology, Sichuan Agricultural University, Ya’an, Sichuan Province 625014, China; 3. Nanchong Professional Technic College, Nanchong, Sichuan Province 637000, China; 4. Agriculture Bureau of Mingshan Area, Mingshan, Sichuan Province 625100, China)
Key Words:FBS; oocytes; IVM; goat
Abstract:This experiment regarded fresh ovary from slaughtered goats as the material. This kind of COC was placed in M199+the FBS(0%, 10%, 15%) educates liquid+FSH(100 IU/mL)+LH(100 IU/mL) educates tiny inside, in the CO2 incubator (5% CO2, 95% air, 100% humidity) under 39 temperature descends to educate 16 h, 24 h and 26 h. It is concluded that 10%~15% FBS is beneficial to the maturation in vitro of goat follicular oocytes. The maturation rate of oocytes cultured for 24 h, 26 h was significantly higher than that of 16 h (P<0.05).
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