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Issue:ISSN 1000-7083
          CN 51-1193/Q
Director:Sichuan Association for Science and Technology
Sponsored by:Sichuan Society of Zoologists; Chengdu Giant Panda Breeding Research Foundation; Sichuan Association of Wildlife Conservation; Sichuan University
Address:College of Life Sciences, Sichuan University, No.29, Wangjiang Road, Chengdu, Sichuan Province, 610064, China
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Your Position :Home->Past Journals Catalog->2014 Vol.33 No.4

Isolation and Identification of Fungi in Feces of Sub-adult Giant Panda
Author of the article:AI Shengquan1#, XIA Yu1#, ZHONG Zhijun1#, WANG chengdong2, LIU Yanhong1, WU Honglin2, LI Caiwu2, YANG Haidi2, LIN Shanshan2, SHI Jinjiang1, PENG Guangneng1*
Author's Workplace:(1. College of Veterinary Medicine, Sichuan Agricultural University/Key Laboratory of Animal Disease and Human Health of Sichuan Province, Ya’an, Sichuan Province 625014, China; 2. Sichuan Ya’an Bifengxia Base, Giant Panda Research Center, Chinese Academy of Forestry Science, Ya’an, Sichuan Province 625007, China)
Key Words:giant panda; intestinal fungus; isolation and identification
Abstract:Objective  To investigate the fungal community structure in the guts of sub-adult giant pandas, and provide the basis for studying the intestinal fungi to digest cellulose and controlling the fungal disease. Methods  In this study, three mediums such as PDA, SDA and Gao’s were chosen to isolate and purify the fungi in fresh feces of sub-adult giant pandas, followed by the combination of morphological and molecular methods for classification. Results  The study showed that the fresh faces of eight sub-adult giant pandas were mainly composed of four kinds of mould (Galactomyces geotrichum, 22.74%; Galactomyces reessii, 12.37%; Mucor ramosissimus, 18.23%; Mucor circinelloides, 6.39%) and two kinds of yeast (Trichosporon sp., 19.46%; Candida solani, 20.81%). The proportions of fungus were different among each sample. The isolation effect of three mediums was: PDA>SDA>Gao. Conclusions  There are a proportion of culturable fungi in the guts of sub-adult giant pandas.
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