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Issue:ISSN 1000-7083
          CN 51-1193/Q
Director:Sichuan Association for Science and Technology
Sponsored by:Sichuan Society of Zoologists; Chengdu Giant Panda Breeding Research Foundation; Sichuan Association of Wildlife Conservation; Sichuan University
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Your Position :Home->Past Journals Catalog->2014 Vol.33 No.3

Long-term Consequences of Paternal Diabetes on Glucose Regulation in the Offspring
Author of the article:PENG Chuan1, XIAO Xiaoqiu2, ZHANG Yuyao2, YU Yang2, LI Jibin1*
Author's Workplace:(1. Department of Nutrition and Food Hygiene, School of Public Health and Management, Chongqing Medical University, Chongqing 400016, China; 2. Laboratory of Lipid and Glucose Research, the First Affiliated Hospital, Chongqing Medical University, Chongqing 400016, China)
Key Words: paternal diabetes; insulin resistance; hepatic gluconeogenesis
Abstract:This study was designed to investigate the long-term consequences of paternal diabeties on the regulation of hepatic gluconeogenesis in the offspring. Diabetic male rat model was established by intraperitoneal injection of streptozotocin (35 mg/kg), the offspring from diabetic male rats bred by female rats was STZ-O group, others from the healthy male rats mated with female rats was CON-O group. The birth weights of the offspring were recorded after delivery, ELISA was conducted to detect the serum insulin level, and glucose tolerance tests (GTT) and insulin tolerance tests (ITT) were measured to evaluate the condition of insulin resistance; pyruvic acid tolerance test (PTT) and real-time PCR were performed to assess the liver gluconeogenesis; periodic acid-schiff stain (PAS) tests was used to assess the liver glycogen deposition. The birth weights of STZ-O group were significantly decreased compared with CON-O group (6.63±0.46 VS 5.72±0.31P=0.0013). There was no difference of random blood glucose level between the two groups, whereas a significant difference was observed in serum insulin level (49.47 μIU/mL±15.63 μIU/mL in STZ-O group and 18.55 μIU/mL±2.59 μIU/mL in CON-O group, P=0.011). There were also significant differences of GTT, ITT, PTT and PAS staining results between the two groups. The expression of two key enzymes involving in hepatic gluconeogenesis, PEPCK and G-6-P, were up-regulated in the STZ-O group. All these results suggested that paternal diabetes would cause long-term consequences including insulin resistance and abnormal activation of hepatic gluconeogenesis on the offspring.
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