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Issue:ISSN 1000-7083
          CN 51-1193/Q
Director:Sichuan Association for Science and Technology
Sponsored by:Sichuan Society of Zoologists; Chengdu Giant Panda Breeding Research Foundation; Sichuan Association of Wildlife Conservation; Sichuan University
Address:College of Life Sciences, Sichuan University, No.29, Wangjiang Road, Chengdu, Sichuan Province, 610064, China
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Your Position :Home->Past Journals Catalog->2014 Vol.33 No.1

Cloning and Expression of Ferritin Heavy Chain Gene in Sichuan White Goose
Author of the article:YI Zhixin, JIANG Dongmei*, KANG Bo*, HE Hui, MA Rong
Author's Workplace:(College of Animal Science and Technology, Sichuan Agricultural University, Ya’an, Sichuan Province 625014, China)
Key Words:Sichuan white goose; ferritin heavy chain; clone; tissue expression
Abstract:Objective  To elucidate the molecular characteristic and expression profiling of ferritin heavy chain (FHC) in various tissues of Sichuan white goose. Methods  The coding sequence of FHC was cloned, as well as the structure and function of FHC was predicted by molecular biological techniques. The expression levels of FHC gene in 13 tissues of Sichuan white goose were determined by quantitative real-time PCR. Results  The length of the coding region of Sichuan white goose FHC gene was 549 bp, encoding 182 amino acids with predicted molecular weight 21345.9 and pI 5.74. The amino acid sequence of goose FHC had the highest similarity with Beijing duck and Gallus gallus (100% and 97%, respectively) in GenBank, and the closest relationship of FHC was observed between Sichuan white goose and Beijing duck. FHC was a stable souble protein, which included 2 highly conserved ferritin iron-binding regions signatures and 7 ferroxidase diiron binding sites. Although FHC was found to be expressed in all the examined tissues, the expression levels were significantly different. The highest expression of FHC was observed in spleen and retina, followed by liver, pituitary, kidney, and muscles. Conclusion  FHC gene of Sichuan white goose is a highly conserved, tissue-specifically gene, which plays a key role in controling the balance of labile iron pool and protecting cells from oxidative stress. This will provide a basis for functional research of FHC gene and the metabolic mechanism of iron in geese.
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