The Effect of Traditional Tibetan Tonic Prescription Basangmu Suyou Wan on the Hematopoiesis Function of Granulocyte-macrophage and the Underlying Mechanism in Radiation, Chemistry Injured Mice
Author of the article:HUANG Xiaoqin1, WEI Lilin2, HUANG Qian1, JIANGYANG Zeren1
Author's Workplace：(1. School of Ethnomedicine, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, China; 2. Affiliated Hospital, Ya’an Vocational College, Ya’an, Sichuan Province 625000, China)
Key Words：Basangmu Suyou Wan; radia-chemotherapy; mice; white blood cell; CFU-GM; GM-CSF
Abstract：Objective To explore the effect of traditional Tibetan medicine tonic prescription Basangmu Suyou Wan on the granulocyte-macrophage hematopoiesis in radiation, chemistry injured mice and elucidate the underlying mechanism. Methods The radiation, chemistry injured mouse model was constructed by 60Coγ ray irradiating and cyclophosphamide peritoneally injecting in normal mice, then the mouse model was fed with different dose of Basangmu Suyou Wan for different time-phases. Subsequently, the changes of white blood cell (WBC) in the peripheral blood, the granulocyte-macrophage hematopoietic progenitors colony forming unit (CFU-GM) proportion, and the granulocyte-macrophage colony stimulating factor (GM-CSF) mRNA as well as the granulocyte-macrophage colony stimulating factor receptor (GM-CSF R) mRNA expression quantity in bone marrow cells (BMC) were determined by blood cell counting, colony forming assaying and fluorescent real-time quantitation PCR. Mouse model without drug feeding (blank group) and injected with physical saline were used as control, respectively. Results After perusing for 14 days, the WBC counts of middle dose and high dose group were significantly higher than the blank and the physical saline group (P <0.05); the BMC of middle dose group had significantly higher CFU-GM proportion, GM-CSF mRNA and GM-CSFR mRNA expression quantity than the blank and physical saline groups (P <0.05). Conclusions Proper dosage of Basangmu Suyou Wan could up-regulate the bone marrow GM-CSF, GM-CSFR mRNA expression, simulate CFU-GM proliferating and differentiating, and thus accelerate the recovery of peripheral WBC level in radiation, chemistry injured mice.